Cyanidin-3-rutinoside attenuates methylglyoxal-induced protein glycation and DNA damage via carbonyl trapping ability and scavenging reactive oxygen species
© The Author(s). 2016
Received: 31 December 2015
Accepted: 18 May 2016
Published: 23 May 2016
Advanced glycation end-products (AGEs) play a significant role in the development and progression of vascular complication in diabetes. Anthocyanin has been recently reported to possess antiglycating activity. This study aimed to determine whether a naturally occurring anthocyanin, cyanidin-3-rutinoside (C3R) inhibits methylglyoxal (MG) induced protein glycation and oxidative protein and DNA damage.
C3R (0.125–1 mM) was incubated with bovine serum albumin and MG (1 mM) for 2 weeks. The formation of fluorescent AGEs was measured by using spectrofluorometer and thiol group content were used to detect protein oxidative damage. Gel electrophoresis was used to determine whether C3R (0.125–1 mM) reduced DNA strand breakage in a glycation model comprising lysine, MG and/or Cu2+. The generation of superoxide anions and hydroxyl radicals were detected by the cytochrome c reduction assay and the thiobarbituric acid reactive substances assay. MG-trapping capacity was assessed by high performance liquid chromatography (HPLC).
C3R (0.25–1 mM) reduced the formation of fluorescent AGEs and depleted protein thiol groups in bovine serum albumin mediated by MG. At 1 mM C3R inhibited oxidative DNA damage in the glycation model (p < 0.05) and at 0.5–1 mM prevented Cu2+ induced DNA strand breakage in the presence of lysine and MG. The findings showed that C3R reduced the formation of superoxide anion and hydroxyl radicals during the glycation reaction of MG with lysine. C3R directly trapped MG in a concentration and time dependent manner (both p < 0.001).
These findings suggest that C3R protects against MG-induced protein glycation and oxidative damage to protein and DNA by scavenging free radicals and trapping MG.
KeywordsCyanidin-3-rutinoside Methylglyoxal Protein glycation Oxidative DNA damage
Methylglyoxal (MG) is recognized as the most reactive glycating agent, generated during glycation and endogenously via carbohydrate, lipid and protein metabolism, especially during the glycolysis pathway [1, 2]. Whilst the glyoxalase defense system converts the damaging MG into D-lactate via the glyoxalase enzyme complex, elevated tissue and plasma levels of MG are commonly observed in diabetes [3, 4]. Methylglyoxal hydroimidazolone (MG-H1) is reported to be the most abundant MG-derived AGE modification in vivo leading to protein dysfunction . The interaction of AGEs with a receptor for AGEs (RAGE) triggers signal transduction by activation of the MAPK pathway, resulting in reactive oxygen species (ROS) overproduction and inflammation . The glycation reaction of amino acids with MG directly generates ROS, causing damage to cellular protein and DNA [7–10]. In particular, oxidative damage of DNA is associated with the development of several pathologies, including physiological ageing, metabolic syndrome, diabetes, cancer and cardiovascular diseases [11–13].
MG has been recognized as a potential target for intervention and novel pharmacological strategies are being developed to limit its accumulation and minimize its detrimental effects. Of these strategies, the ability for some AGE inhibitors to trap MG has received considerable interest . The early clinical trials with aminoguanidine (AG) showed great promise as an AGEs inhibitor. However, studies in people with diabetic nephropathy were terminated following safety and efficacy concerns [15, 16]. Alternatively, studies on AGE inhibitors from natural products show more promise to combat AGE-associated diseases by scavenging free radicals, or by directly trapping MG. In this regard, pyridoxamine, a form of naturally occurring vitamin B6 and inducer of glyoxalase enzyme expression -isothiocyanates and sulforaphanes found in cruciferous vegetables, have been reported to have some promise [17, 18]. In particular, anthocyanins, the colourful pigment in various fruits and vegetables reduced ROS generation in human HepG2 cells exposed to a high glucose environment . This could explain, in part their biological effectiveness as anti-oxidant, anti-carcinogenic, anti-microbial and anti-inflammatory agents and their role in ameliorating hyperglycemia by improving insulin sensitivity via the cAMP-activated protein kinase pathway in diabetic mice [20–23]. A derivative of anthocyanin, cyanidin-3-rutinoside (C3R), maybe particularly important as it delays postprandial glycemia by inhibiting α-glucosidase and pancreatic α-amylase which play an important role in glucose metabolism [24–26]. Most recently, C3R inhibited ribose-, fructose-, glucose- and galactose-induced protein glycation and oxidation in vitro . Collectively these findings suggest that C3R may prevent MG-induced AGEs formation and oxidative protein and DNA damage which have not been investigated previously.
This study aimed to determine whether C3R inhibited MG-induced protein glycation (formation of AGEs) and oxidation (depletion of thiol groups) in vitro, and reduced MG/lysine-induced DNA damage using a plasmid DNA assay. To investigate possible mechanisms of action, we evaluated the role of C3R in reducing the generation of superoxide anion and hydroxyl radicals as well as direct scavenging of the MG. Due to the complexity of the glycation cascade, in vitro models were used as they enabled identification of the pathways of action.
Chemicals and Reagents
Bovine serum albumin (BSA) fraction V was purchased from Fisher scientific (Hudson, NH, USA). 40 % solution methylglyoxal (MG), 5,5′-dithiobis (2-nitrobenzoic acid) (DTNB), o-phenylenediamine (o-PD), 5-methylquinoxaline (5-MQ), 2-deoxy-D-ribose, 2-thiobarbituric acid (TBA), L-cysteine and aminoguanidine hydrochloride (AG) were purchased from Sigma (St. Louis, MO, USA). L-lysine hydrochloride was purchased from Himedia (L.B.S. Marg, MB, India). Trichloroacetic acid, methanol (gradient grade for liquid chromatography) and guanidine hydrochloride were purchased from Merck (Darmstadt, Germany). Cytochrome c was obtained from Affymetrix (Santa Clara, CA, USA). Cyanidin-3-rutinoside chloride was synthesized from quercetin-3-rutinoside according to a previous study .
Bovine serum albumin (BSA)-methylglyoxal assay
FC was fluorescence intensity of MG with BSA and FCB were the fluorescence intensity of blank. FS and FSB were the fluorescence intensity of C3R or AG with BSA/MG and blank, respectively.
Determination of thiol group content
Thiol groups in glycated BSA were measured after 1 and 2 weeks of incubation according to the Ellman assay . Briefly, BSA samples (10 μL) were mixed with 2.5 mM DTNB (90 μL) for 15 min. The absorbance was measured at 412 nm. The free thiol concentration was calculated by using a standard curve of L-cysteine.
Analysis of DNA strand breaks
Determination of superoxide anions
The level of superoxide anions was determined by measurement of cytochrome c reduction assay according to a previous method . In brief, 10 mM lysine and 10 mM MG was incubated in the presence or absence of C3R at concentration 0.125–1 mM. The reduction rate of cyctochrome c was measured at room temperature using a spectrophotometer at 550 nm at 10 min intervals for 50 min. The level of reduced cytochrome c was calculated based on the extinction coefficient for cytochrome c (ε = 27,700 M−1cm−1).
Determination of hydroxyl radicals
Thiobarbituric acid reactive substances (TBARS) assay was used to evaluate the level of hydroxyl radicals according to a previously described method . Briefly, the reaction mixture (0.2 mL total volume) containing 10 mM lysine, 10 mM MG, and 20 mM 2-deoxy-d-ribose was incubated at 37 °C with or without C3R (0.125–1 mM). After 3 h, 0.2 mL PBS and 0.2 mL TCA (2.8 % w/v) was added to the reaction mixture, followed by 0.2 mL thiobarbituric acid (TBA). The solution was boiled at 100 °C for 10 min and then cooled to room temperature. The degradation of 2-deoxy-d-ribose was measured at a wavelength 532 nm using a spectrophotometer. Hydroxyl radicals were expressed as the level of TBARS which was quantified by using standard curve of malondialdehyde.
Determination of MG-trapping ability
Data are presented as the arithmetic means ± SEM for each treatment group (n = 3). The effect of C3R on the formation of AGEs, protein oxidation, DNA strand break and MG-induced generation of hydroxyl radical was analyzed by one-way ANOVA. The effect of C3R on MG-induced generation of superoxide anion was determined by two-way repeated measures ANOVA. The effect of C3R on MG-trapping ability was determined by a two-way ANOVA. Differences between treatments were analyzed by a Tukey post hoc test. These analyses were performed using SPSS Statistics 17.0 (SPSS Inc., Chicago, IL, USA). A P value of less than 0.05 was taken as the criterion of significance.
C3R and the formation of AGEs
C3R and protein oxidation
C3R and MG-induced DNA strand breakage
C3R and MG-induced generation of superoxide anion and hydroxyl radicals
The lysine/MG induced generation of hydroxyl radicals (as measured by TBARS) was reduced when C3R was added at the level of 0.5 mM or above (Fig. 5b). The highest concentration of C3R (1 mM) resulted in a 20 % inhibition of hydroxyl radical generation.
C3R and MG trapping ability
The percentage of methylglyoxal (MG)-trapping ability of cyanidin-3-rutinoside (C3R) and aminoguanidine (AG)
Molar ratio of C3R:MG
Molar ration of AG:MG
5 ± 0.3
12 ± 1.3
21 ± 2.6
32 ± 2.0
42 ± 2.5
61 ± 1.9
90 ± 3.2
9 ± 1.2
21 ± 1.0
45 ± 0.7
57 ± 3.0
73 ± 3.2
89 ± 1.1
95 ± 4.6
The development of macrovascular and microvascular diabetic complications is associated with the formation and accumulation of advanced glycation end products (AGEs) [33, 34]. There is an important need to identify approaches that may prevent their formation [35, 36] and this study shows for the first time that cyanidin-3-rutinoside (C3R) effectively inhibited dicarbonyl intermediate methylglyoxal (MG)-derived AGEs formation at an immediate stage of the glycation process. C3R finally suppressed the formation of fluorescent AGEs and non-fluorescent AGEs in advanced stage of glycation process. This inhibitory activity of C3R on protein glycation was just as effective as aminoguanidine (AG) when highest dose (1 mM) was provided. These findings extend previous research which showed that C3R inhibited ribose-, fructose-, glucose- and galactose-induced AGEs formation during the initial stage of glycation associated with the reduction of Amadori product fructosamine . Furthermore, berry and grape extracts containing anthocyanin also inhibited the formation of AGEs in an in vitro model involving fructose, MG and BSA . It is likely that these protein glycation protective effects of the fruit extracts may be largely due to C3R content as it is the predominant anthocyanin component [38, 39]. These findings, taken together, suggest that C3R potentially impairs initiation and intermediate stages of protein glycation and supports the need for efficacy and bioavailability studies of C3R in animals and humans.
The effectiveness of C3R in reducing the formation of AGEs can be explained in part by the effect of C3R on scavenging superoxide anion and hydroxyl radicals. In the current study, we showed that in the presence of lysine and MG, C3R reduced the level of TBARS formation at concentrations of 0.25 mM or greater and maintained the reduced form of cytochrome c at background levels. C3R also prevented the depletion of thiol, a marker of protein oxidation with higher potency than AG when provided at an equal concentration (1 mM) in BSA/MG (1 mM) system. In previous report, the ROS production during glycation process in BSA/MG system showed potent to induce DNA strand breakage when used the higher concentration of MG (20 mM) for 10 and 21 days of incubation . Protein cross-linking mediated by MG generates free radicals during this reaction including the MG-radical anion and cross-linked radical cation (MG-protonated cation) . The MG-protonated cation is a precursor of fluorescent AGEs while MG-radical anions could donate an electron to oxygen molecule to generate a superoxide anion and hydroxyl radical [9, 10, 42]. The presence of a transition metal ion copper could stimulate the Fenton-like reaction to produce more highly reactive hydroxyl radicals that play an important role in mechanism of oxidative DNA strand breakage . The ability of phytochemical compounds on the prevention of MG-induced protein glycation and DNA damage related to free radical scavenging activity has been reported [31, 42, 43]. Anthocyanin-rich extract containing high concentration of C3R also exhibits potent antioxidant activity [44, 45] and it is suggested that C3R may act as free radical scavenger to prevent oxidative damage of protein and DNA. These studies support previous reports that C3R inhibited the production of a secondary product of oxidation malonaldehyde from the degradation of 2-deoxyribose unit in DNA mediated by Fenton’s reagent as well as decreased ROS production and DNA damage in hydrogen peroxide-simulated RAW 264.7 murine macrophage cell [46, 47].
The current study demonstrated that C3R was able to directly trap MG which explains, in part its action in preventing the formation of AGEs and oxidative protein and DNA damage. The percentage of MG-trapping ability increased in a concentration dependent manner and these findings are supported by a previous study which showed that a purified C3R from blackcurrant extract trapped nearly 50 % of MG when it was incubated at a 1:1 ratio. The C3R-mono-MG adduct was identified as product from the reaction by using liquid chromatography electrospray ionization mass spectrometer (LC-ESI-MS) . The molecular weight difference between the adduct (667 m/z) and the original C3R (595 m/z) is 72 which is same as the molecular weight of one molecule of MG. It has been reported the addition of MG on anthocyanin may probably form tautomers with transformation of carbonyl group of MG to hydroxyl group . In the previous study, the MG addition reaction reacted with phenolic compounds at carbon atom with negative electron charge more than −0.24. Therefore, carbon number 2, 6, 7 and 15 of C3R is the possible location for MG addition reaction . Chen et al. demonstrated the C3R/MG reaction for only 1 h  but the different molar ratio and incubation time between C3R and MG was evaluated in the current study. The reaction rate between C3R and MG at 1:1 molar ratio was nearly 2-fold higher when the incubation time was increased from 1 to 24 h. It suggests that the trapping ability of C3R is dependent on the concentration and time of incubation. In addition, flavonoids have also been shown to inhibit AGEs formation via MG-trapping ability [37, 50–53] which may be due to their chemical structure that consists of phenyl ring (A- and B-ring) and heterocyclic ring (C-ring). The major sites to conjugate with MG are the carbon positions at 6 and 8 on the A-ring  which is the same structure present in C3R and may explain their effectiveness in trapping MG .
2-MQ, 2-methylquinoxaline; AG, Aminoguanidine; AGEs, Advanced glycation end products; BSA, Bovine serum albumin; C3R, Cyanidin-3-rutinoside; MG, Methylglyoxal; ROS, Reactive oxygen species; TBAR, Thiobarbituric acid reactive substances.
We would like to acknowledge the excellent technical assistance provided by Dr. Weerachat Sompong.
Thavaree Thilavech was financially supported by RGJ-PhD program (PHD/0027/2554), the Thailand Research Fund (TRF) and Chulalongkorn University and the 90th Anniversary of Chulalongkorn University Fund (Ratchadaphiseksomphot Endowment Fund). This research was supported by Grant for International Research Integration: Chula Research Scholar, Ratchadaphiseksomphot Endowment Fund.
Availability of data and materials
Data are all contained within the paper.
TT was responsible for the study design, experiments, acquisition of data, analysis and interpretation of data, drafting manuscript and revision. SN was collaborated for the study and writing concept. DB, MA participated in the writing concept and drafted manuscript. SA contributed concept of the study, drafted and revised manuscript. All authors read and approved the final version of manuscript to be published.
The authors declare that they have no competing interests.
Consent for publication
Ethic approval and consent to participate
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Kalapos MP. Methylglyoxal in living organisms: chemistry, biochemistry, toxicology and biological implications. Toxicol Lett. 1999;110(3):145–75.View ArticlePubMedGoogle Scholar
- Nowotny K, Jung T, Hohn A, Weber D, Grune T. Advanced glycation end products and oxidative stress in type 2 diabetes mellitus. Biomolecules. 2015;5(1):194–222.View ArticlePubMedPubMed CentralGoogle Scholar
- Ogawa S, Nakayama K, Nakayama M, Mori T, Matsushima M, Okamura M, et al. Methylglyoxal is a predictor in type 2 diabetic patients of intima-media thickening and elevation of blood pressure. Hypertension. 2010;56(3):471–6.View ArticlePubMedGoogle Scholar
- Phillips SA, Mirrlees D, Thornalley PJ. Modification of the glyoxalase system in streptozotocin-induced diabetic rats. Effect of the aldose reductase inhibitor Statil. Biochem Pharmacol. 1993;46(5):805–11.View ArticlePubMedGoogle Scholar
- Schalkwijk CG. Vascular AGE-ing by methylglyoxal: the past, the present and the future. Diabetologia. 2015;58(8):1715–9.View ArticlePubMedPubMed CentralGoogle Scholar
- Singh VP, Bali A, Singh N, Jaggi AS. Advanced glycation end products and diabetic complications. Korean J Physiol Pharmacol. 2014;18(1):1–14.View ArticlePubMedPubMed CentralGoogle Scholar
- An SH, Kang JH. Oxidative damage of DNA induced by the reaction of methylglyoxal with lysine in the presence of ferritin. BMB Rep. 2013;46(4):225–9.View ArticlePubMedPubMed CentralGoogle Scholar
- Argirova M, Breipohl W. Comparison between modifications of lens proteins resulted from glycation with methylglyoxal, glyoxal, ascorbic acid, and fructose. J Biochem Mol Toxic. 2002;16(3):140–5.View ArticleGoogle Scholar
- Kang JH. Oxidative damage of DNA induced by methylglyoxal in vitro. Toxicol Lett. 2003;145(2):181–7.View ArticlePubMedGoogle Scholar
- Suji G, Sivakami S. DNA damage during glycation of lysine by methylglyoxal: assessment of vitamins in preventing damage. Amino Acids. 2007;33(4):615–21.View ArticlePubMedGoogle Scholar
- Cooke MS, Evans MD, Dizdaroglu M, Lunec J. Oxidative DNA damage: mechanisms, mutation, and disease. FASEB J. 2003;17(10):1195–214.View ArticlePubMedGoogle Scholar
- Malik Q, Herbert KE. Oxidative and non-oxidative DNA damage and cardiovascular disease. Free Radic Res. 2012;46(4):554–64.View ArticlePubMedGoogle Scholar
- Hoeijmakers JH. DNA damage, aging, and cancer. N Engl J Med. 2009;361(15):1475–85.View ArticlePubMedGoogle Scholar
- Rahbar S, Figarola JL. Novel inhibitors of advanced glycation endproducts. Arch Biochem Biophys. 2003;419(1):63–79.View ArticlePubMedGoogle Scholar
- Bolton WK, Cattran DC, Williams ME, Adler SG, Appel GB, Cartwright K, et al. Randomized trial of an inhibitor of formation of advanced glycation end products in diabetic nephropathy. Am J Nephrol. 2004;24(1):32–40.View ArticlePubMedGoogle Scholar
- Freedman BI, Wuerth JP, Cartwright K, Bain RP, Dippe S, Hershon K, et al. Design and baseline characteristics for the aminoguanidine clinical trial in overt type 2 diabetic nephropathy (ACTION II). Control Clin Trials. 1999;20(5):493–510.View ArticlePubMedGoogle Scholar
- Xue M, Rabbani N, Momiji H, Imbasi P, Anwar MM, Kitteringham N, et al. Transcriptional control of glyoxalase 1 by Nrf2 provides a stress-responsive defence against dicarbonyl glycation. Biochem J. 2012;443(1):213–22.View ArticlePubMedGoogle Scholar
- Nagaraj RH, Sarkar P, Mally A, Biemel KM, Lederer MO, Padayatti PS. Effect of pyridoxamine on chemical modification of proteins by carbonyls in diabetic rats: characterization of a major product from the reaction of pyridoxamine and methylglyoxal. Arch Biochem Biophys. 2002;402(1):110–9.View ArticlePubMedGoogle Scholar
- Zhu W, Jia Q, Wang Y, Zhang Y, Xia M. The anthocyanin cyanidin-3-O-beta-glucoside, a flavonoid, increases hepatic glutathione synthesis and protects hepatocytes against reactive oxygen species during hyperglycemia: Involvement of a cAMP-PKA-dependent signaling pathway. Free Radic Biol Med. 2012;52(2):314–27.View ArticlePubMedGoogle Scholar
- Takikawa M, Inoue S, Horio F, Tsuda T. Dietary anthocyanin-rich bilberry extract ameliorates hyperglycemia and insulin sensitivity via activation of AMP-activated protein kinase in diabetic mice. J Nutr. 2010;140(3):527–33.View ArticlePubMedGoogle Scholar
- Bagchi D, Sen CK, Bagchi M, Atalay M. Anti-angiogenic, antioxidant, and anti-carcinogenic properties of a novel anthocyanin-rich berry extract formula. Biochem Mosc. 2004;9(1):75–80.View ArticleGoogle Scholar
- He J, Giusti MM. Anthocyanins: natural colorants with health-promoting properties. Annu Rev Food Sci Technol. 2010;1:163–87.View ArticlePubMedGoogle Scholar
- Wallace TC. Anthocyanins in cardiovascular disease. Adv Nutr. 2011;2(1):1–7.View ArticlePubMedPubMed CentralGoogle Scholar
- Adisakwattana S, Yibchok-Anun S, Charoenlertkul P, Wongsasiripat N. Cyanidin-3-rutinoside alleviates postprandial hyperglycemia and its synergism with acarbose by inhibition of intestinal alpha-glucosidase. J Clin Biochem Nutr. 2011;49(1):36–41.View ArticlePubMedPubMed CentralGoogle Scholar
- Akkarachiyasit S, Yibchok-Anun S, Wacharasindhu S, Adisakwattana S. In vitro inhibitory effects of cyandin-3-rutinoside on pancreatic alpha-amylase and its combined effect with acarbose. Molecules. 2011;16(3):2075–83.View ArticlePubMedGoogle Scholar
- Matsui T, Ebuchi S, Kobayashi M, Fukui K, Sugita K, Terahara N, et al. Anti-hyperglycemic effect of diacylated anthocyanin derived from Ipomoea batatas cultivar Ayamurasaki can be achieved through the alpha-glucosidase inhibitory action. J Agric Food Chem. 2002;50(25):7244–8.View ArticlePubMedGoogle Scholar
- Thilavech T, Ngamukote S, Abeywardena M, Adisakwattana S. Protective effects of cyanidin-3-rutinoside against monosaccharides-induced protein glycation and oxidation. Int J Biol Macromol. 2015;75:515–20.View ArticlePubMedGoogle Scholar
- Elhabiri M, Figueiredo P, Fougerousse A, Brouillard R. A convenient method for conversion of flavonols into anthocyanins. Tetrahedron Lett. 1995;36(26):4611–4.View ArticleGoogle Scholar
- Meeprom A, Sompong W, Chan CB, Adisakwattana S. Isoferulic Acid, a new anti-glycation agent, inhibits fructose- and glucose-mediated protein glycation in vitro. Molecules. 2013;18(6):6439–54.View ArticlePubMedGoogle Scholar
- Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys. 1959;82(1):70–7.View ArticlePubMedGoogle Scholar
- Chan WH, Wu HJ. Protective effects of curcumin on methylglyoxal-induced oxidative DNA damage and cell injury in human mononuclear cells. Acta Pharmacol Sin. 2006;27(9):1192–8.View ArticlePubMedGoogle Scholar
- Peng X, Zheng Z, Cheng K-W, Shan F, Ren G-X, Chen F, et al. Inhibitory effect of mung bean extract and its constituents vitexin and isovitexin on the formation of advanced glycation endproducts. Food Chem. 2008;106(2):475–81.View ArticleGoogle Scholar
- Goldin A, Beckman JA, Schmidt AM, Creager MA. Advanced glycation end products: sparking the development of diabetic vascular injury. Circulation. 2006;114(6):597–605.View ArticlePubMedGoogle Scholar
- Jakus V, Rietbrock N. Advanced glycation end-products and the progress of diabetic vascular complications. Physiol Res. 2004;53(2):131–42.PubMedGoogle Scholar
- Yamagishi S, Nakamura K, Imaizumi T. Advanced glycation end products (AGEs) and diabetic vascular complications. Curr Diabetes Rev. 2005;1(1):93–106.View ArticlePubMedGoogle Scholar
- Wu CH, Huang SM, Lin JA, Yen GC. Inhibition of advanced glycation endproduct formation by foodstuffs. Food Funct. 2011;2(5):224–34.View ArticlePubMedGoogle Scholar
- Wang W, Yagiz Y, Buran TJ, Nunes CN, Gu L. Phytochemicals from berries and grapes inhibited the formation of advanced glycation end‐products by scavenging reactive carbonyls. Food Res Int. 2011;44(9):2666–73.View ArticleGoogle Scholar
- Hassimotto NM, Genovese MI, Lajolo FM. Absorption and metabolism of cyanidin-3-glucoside and cyanidin-3-rutinoside extracted from wild mulberry (Morus nigra L.) in rats. Nutr Res. 2008;28(3):198–207.View ArticlePubMedGoogle Scholar
- Jakobek L, Šeruga M, Medvidović-Kosanović M, Jovanović IN. Anthocyanin content and antioxidant activity of various red fruit juices. Dtsch Lebensmitt Rundsch. 2007;103(2):58–64.Google Scholar
- Ali A, Sharma R. Acomparative study on the role of lysine and BSA in glycation-induced damage to DNA. Biosci Bioeng Commun. 2015;1:38–43.Google Scholar
- Yim HS, Kang SO, Hah YC, Chock PB, Yim MB. Free radicals generated during the glycation reaction of amino acids by methylglyoxal. A model study of protein-cross-linked free radicals. J Biol Chem. 1995;270(47):28228–33.View ArticlePubMedGoogle Scholar
- Wu CH, Yen GC. Inhibitory effect of naturally occurring flavonoids on the formation of advanced glycation endproducts. J Agric Food Chem. 2005;53(8):3167–73.View ArticlePubMedGoogle Scholar
- Meeprom A, Sompong W, Suantawee T, Thilavech T, Chan CB, Adisakwattana S. Isoferulic acid prevents methylglyoxal-induced protein glycation and DNA damage by free radical scavenging activity. BMC Complement Altern Med. 2015;15(1):34.View ArticleGoogle Scholar
- Li W, Liang H, Zhang MW, Zhang RF, Deng YY, Wei ZC, et al. Phenolic profiles and antioxidant activity of litchi (Litchi Chinensis Sonn.) fruit pericarp from different commercially available cultivars. Molecules. 2012;17(12):14954–67.View ArticlePubMedGoogle Scholar
- Beaulieu LP, Harris CS, Saleem A, Cuerrier A, Haddad PS, Martineau LC, et al. Inhibitory effect of the Cree traditional medicine wiishichimanaanh (Vaccinium vitis-idaea) on advanced glycation endproduct formation: identification of active principles. Phytother Res. 2010;24(5):741–7.PubMedGoogle Scholar
- Jung H, Kwak H-K, Hwang K. Antioxidant and antiinflammatory activities of cyanidin-3-glucoside and cyanidin-3-rutinoside in hydrogen peroxide and lipopolysaccharide-treated RAW264.7 cells. Food Sci Biotechnol. 2014;23(6):2053–62.View ArticleGoogle Scholar
- Matsufuji H, Ochi H, Shibamoto T. Formation and inhibition of genotoxic malonaldehyde from DNA oxidation controlled with EDTA. Food Chem Toxicol. 2006;44(2):236–41.View ArticlePubMedGoogle Scholar
- Chen X-Y, Huang IM, Hwang LS, Ho C-T, Li S, Lo C-Y. Anthocyanins in blackcurrant effectively prevent the formation of advanced glycation end products by trapping methylglyoxal. J Funct Foods. 2014;8:259–68.View ArticleGoogle Scholar
- Lv L, Shao X, Wang L, Huang D, Ho CT, Sang S. Stilbene glucoside from Polygonum multiflorum thunb.: A novel natural inhibitor of advanced glycation end product formation by trapping of methylglyoxal. J Agric Food Chem. 2010;58(4):2239–45.View ArticlePubMedGoogle Scholar
- Hu TY, Liu CL, Chyau CC, Hu ML. Trapping of methylglyoxal by curcumin in cell-free systems and in human umbilical vein endothelial cells. J Agric Food Chem. 2012;60(33):8190–6.View ArticlePubMedGoogle Scholar
- Li X, Zheng T, Sang S, Lv L. Quercetin inhibits advanced glycation end product formation by trapping methylglyoxal and glyoxal. J Agric Food Chem. 2014;62(50):12152–8.View ArticlePubMedGoogle Scholar
- Peng X, Cheng KW, Ma J, Chen B, Ho CT, Lo C, et al. Cinnamon bark proanthocyanidins as reactive carbonyl scavengers to prevent the formation of advanced glycation endproducts. J Agric Food Chem. 2008;56(6):1907–11.View ArticlePubMedGoogle Scholar
- Sang S, Shao X, Bai N, Lo CY, Yang CS, Ho CT. Tea polyphenol (−)-epigallocatechin-3-gallate: a new trapping agent of reactive dicarbonyl species. Chem Res Toxicol. 2007;20(12):1862–70.View ArticlePubMedGoogle Scholar