Fig. 6

Morphological and cytoskeletal alterations of MG-63 cells analyzed by different microscopic techniques. a Morphological alterations of MG-63 cells after treatment with DMSO (control) or 100 μg/ml VSM and BORM by bright field imaging (upper panel) and scanning electron microscopy (lower panel). b Calculation of the cell size quotients by length and width measurements of the cells in the scanning electron micrographs (left). n = 30. Enlarged section of BORM treated MG-63 cells. Distinctly, the vesicles accumulation can be detected around cell nucleus (right). c Laser scanning microscopic images of F-actin (red) and ß-tubulin (green) stained MG-63 cells. Cells were counterstained with Hoechst to label the cell nuclei (blue). Notably, VSM induced an increased Actin stress fiber formation through the entire cell, leading to a greater cell surface area. In contrast, treatment with BORM caused an increased production of vesicle-like structures and a spindle-shaped cell shape. Magnification bars = 20 μm