Fig. 3

Effects of CSYJE on LPS-stimulated production of pro-inflammatory mediators in RAW 264.7 cells. Levels of NO (a), PGE₂ (b), and IL-6 (c) were measured in the culture supernatants from cells treated with CSYJE and LPS. Cells were pretreated with CSYJE (250, 500, or 1000 μg/mL) for 4 h and LPS (1 μg/mL) for additional 20 h for NO, PGE₂, and IL-6 assays. Cells were pretreated with CSYJE (250, 500, or 1000 μg/mL for NO and IL-6 assays, and 15.625, 31.25, 62.5, 125, 250, 500, or 1000 μg/mL for PGE₂ assay) for 4 h and LPS (1 μg/mL) for additional 20 h for PGE₂ assay. L-N-methylarginine (L-NMMA; (100 μM) and indomethacin (2.5 ng/mL) were used as positive control drugs. The bar graphs represent the means from three independent experiments. ^##P < 0.01 vs vehicle control cells; and **P < 0.01 vs LPS-treated cells