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Chungsimyeonja-eum inhibits inflammatory responses in RAW 264.7 macrophages and HaCaT keratinocytes
© Lim et al. 2015
Received: 7 July 2015
Accepted: 7 October 2015
Published: 16 October 2015
Chungsimyeonja-eum (CSYJE) is an herbal prescription used in traditional Oriental medicine for treating cerebral infarction by reducing ischemic damage. However, the effects of CSYJE on inflammation have not been verified scientifically.
Anti-inflammatory effects of CSYJE was investigated to dertermine the inhibitory effects of CSYJE against inflammation using RAW 264.7 mouse macrophages and HaCaT human keratinocytes. To measure the effects of CSYJE on inflammatory mediators and cytokines/chemokines, we used the following methods: cell viability assay, enzyme-linked immunosorbent assay (ELISA), western blotting, immunocytochemistry. RAW 264.7 cells were pretreated with CSYJE (250, 500, or 1000 μg/mL) for 4 h and treated with lipopolysaccharide (LPS) for additional 20 h. HaCaT cells were stimulated with tumor necrosis factor alpha (TNF-α) and interferon gamma (IFN-γ) (TI), and CSYJE (125, 250, or 500 μg/mL) for 24 h.
CSYJE suppressed the production of nitric oxide (NO, IC50 1000 μg/mL), prostaglandin E2 (PGE2, IC50 = 12.1 μg/mL), and interleukin (IL)-6 (IC50 = 248 μg/mL) in LPS-stimulated RAW 264.7 cells. CSYJE suppressed the effects of TI on the production of thymus and activation-regulated chemokine (TARC, IC50 = 330.2 μg/mL), macrophage-derived chemokine (MDC/CCL22, IC50 = 52.5 μg/mL), regulated on activation, normal T-cell expressed and secreted (RANTES/CCL5, IC50 = 372.9 μg/mL), and IL-8 (IC50 = 345.1 μg/mL) in HaCaT cells. CSYJE inhibited TI-stimulated STAT1 phosphorylation in a dose-dependent manner and nuclear translocation at 500 μg/mL in HaCaT cells.
Our results suggest a possible therapeutic application of CSYJE for treating inflammatory diseases.
Inflammation constitutes multiple processes, characterized by the infiltration of inflammatory cells, such as Th2-type cells, eosinophils, mast cells, and macrophages. Of these activated cells, macrophages play a central role in managing many different pathological immune phenomena such as the over-production of inflammatory mediators. Various mediators, such as nitric oxidase, prostaglandins, and inflammatory cytokines such as interleukin (IL)-6, IL-1β and tumor necrosis factor (TNF)-α, and others induced by macrophages have important roles in inflammatory diseases [1–3].
Migration of inflammatory cells is regulated by chemokines. These are small proteins released from various cell types, which regulate the traffic of immune cells to site of inflammation or infection. Various inflammatory cytokines stimulate the expression of inflammatory chemokines [4, 5]. Among them, thymus and activation-regulated chemokine (TARC/CCL17), macrophage-derived chemokine (MDC/CCL22), and regulated on activation, normal T-cell expressed and secreted (RANTES/CCL5) are typical inflammatory chemokines and ligands for CC chemokine receptor 4 (CCR4), which is predominantly expressed on keratinocytes, Th2 lymphocytes, and basophils [6, 7]. In addition, IL-8 is another inflammatory mediator involved in allergic responses that is closely associated with the severity of chronic inflammation .
Extracellular binding of cytokines induces activation of the intracellular Janus kinase (JAK) that phosphorylates a specific tyrosine residue in signal transducer and activator of transcription (STAT) proteins , which are involved in the development and function of the immune system . There are seven mammalian STAT family members that have been identified: STAT1, STAT2, STAT3, STAT4, STAT5 (STAT5A and STAT5B) and STAT6. In particular, STAT1 is a crucial molecule for the interferon (IFN)/cytokine-signaling pathways . Thus, IFN-γ stimulates JAK phosphorylation, which in turn induces STAT1 phosphorylation . These events eventually increase the production of chemokines including TARC, MDC, and RANTES [13, 14].
The traditional herbal formula Chungsimyeonja-eum (CSYJE) comprises nine medicinal herbs and has traditionally been used in the treatment of heart and brain diseases in Oriental medicine. Previous research reported that the water extract of CSYJE has cytoprotective effect against glutamate-induced C6 glial cell death . Other studies showed that CSYJE protects against ischemic stroke and has anti-stress activity [16, 17]. However, there has been no investigation focusing on the inflammatory effects of CSYJE. Therefore, we investigated the effects and action mechanisms of CSYJE on the inflammatory mediators in RAW 264.7 macrophages and HaCaT keratinocytes.
Preparation of CSYJE water extract
Herbal composition of a single dose of CSYJE
Nelumbo nucifera Gaerhner
Poria cocos Wolf
Panax ginseng C. A. Meyer
Astragalus membranaceus Bunge
Scutellaria baicalensis Georgi
Plantago asiatica L.
Liriope platyphylla Wang et Tang
Lycii Radicis Cortex
Lycium chinense Miller
Glycyrrhizae Radix et Rhizoma
Glycyrrhiza uralensis Fischer
High-performance liquid chromatography (HPLC) analysis of CSYJE
Quantitative analysis of the CSYJE sample was performed using a Shimadzu LC-20A Prominence HPLC system (Shimadzu Co., Kyoto, Japan) consisting of a solvent delivery unit, an on-line degasser, a column oven, an autosampler, and a photo diode array (PDA) detector. The data were acquired and processed by LabSolution software (Version 1.24 SP1). A SunFire C18 column (250 × 4.6 mm; particle size 5 μm, Waters, Milford, MA, USA), which was maintained at 40 °C, was used as the stationary phase and the mobile phases were consisted of 0.1 % (v/v) formic acid in water (A) and 0.1 % (v/v) formic acid in acetonitrile (B). The elution conditions were as follows: 10–60 % B for 0–30 min, 60–100 % B for 30–40 min, 100 % B for 40–45 min, and 100–10 % B for 45–50 min. The flow-rate was 1.0 mL/min and injection volume was 10 μL. For HPLC analysis of CSYJE, lyophilized 200 mg of CSYJE extract was dissolved in 20 mL of distilled water and then the solution was diluted to 10-fold for quantitative analysis of baicalin and wogonoside. Each solution was filtered through a SmartPor GHP 0.2 μm syringe filter (Woongki Science, Seoul, Korea) before HPLC injection.
Murine macrophage RAW 264.7 and human keratinocyte HaCaT were obtained from the American Type Culture Collection (Rockville, MD) and CLS Cell Lines Service GmbH (Eppelheim, Baden-Württemberg, Germany), respectively. The cells were cultured in Dulbecco’s modified Eagle’s medium (Gibco Inc., Grand Island, NY) supplemented with 5.5 % (for RAW 264.7) or 10 % (for HaCaT) heat-inactivated fetal bovine serum (Gibco Inc.), penicillin (100 U/mL), and streptomycin (100 μg/mL) in a 5 % CO2 incubator at 37 °C.
Cell viability was assessed using a Cell Counting Kit-8 assay (CCK-8 from Dojindo, Kumamoto, Japan) according to the manufacturer’s instructions. RAW 264.7 cells (3 × 103 cells/well) and HaCaT cells (1 × 103 cells/well) were incubated in 96-well plates with various concentrations of the CSYJE for 24 h. The CCK-8 reagent was added to each well, followed by incubation for an additional 4 h, and the absorbance was measured at 450 nm using a Benchmark plus microplate reader (Bio-Rad Laboratories, Hercules, CA). The percentage of viable cells was calculated using the following equation: cell viability (%) = (mean absorbance in test well/mean absorbance in control well) × 100.
Measurement of nitric oxide (NO), prostaglandin E2 (PGE2), and IL-6 production
RAW 264.7 cells were plated at a density of 2.5 × 105 cells/well in 48-well plates and incubated overnight. Cells were pretreated with various concentrations of CSYJE for 4 h and then treated with LPS (1 μg/mL) for an additional 20 h. The supernatants were collected and analyzed for the levels of NO (Griess Reagent System; Promega Corp., Madison, WI), and PGE2 and IL-6 using enzyme-linked immunosorbent assay (ELISA) kits (Cayman Chemical Co., Ann Arbor, MI for PGE2, and R&D Systems, Minneapolis, MN) according to the manufacturers’ protocols.
Measurement of chemokine production
HaCaT cells (1 × 106 cells/well) were cultured in 6-well plates. After reaching confluency, the cells were washed and treated with CSYJE in 1 mL of serum-free medium containing tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) (TI, each 10 ng/mL; R&D Systems Inc., Minneapolis, MN, USA) for 24 h. The production of TARC, MDC, RANTES, and IL-8 was determined using commercial ELISA kits (R&D Systems Inc.).
HaCaT cells were treated with a nontoxic concentration of CSYJE for 1 h, and then incubated in the presence of TI for 30 min. The cells were collected by centrifugation, washed twice with PBS, and suspended in an extraction lysis buffer (Sigma-Aldrich, St. Louis, MO) containing protease inhibitors. The protein concentration was determined using a protein assay reagent (Bio-Rad Laboratories, Hercules, CA) according to the manufacturer’s instructions. Equal amounts of total protein (30 μg) were resolved by 10 % sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a nitrocellulose membrane. The membrane was incubated with blocking solution [5 % skim milk in Tris-buffered saline containing Tween 20 (TBST)], followed by an overnight incubation at 4 °C with the appropriate primary antibody. The following primary antibodies and dilution were used: anti-β-actin (1:1000 dilution; Cell Signaling, Danvers, MA), anti-STAT1, and anti-phospho-STAT1 (1:1000 dilution; Abcam, Cambridge, UK). The membranes were washed three times with TBST, and then incubated with a 1:3000 dilution of a horseradish peroxidase (HRP)-conjugated secondary antibody (Jackson ImmunoResearch, West Grove, PA) for 1 h at room temperature. The membranes were again washed three times with TBST, and then developed using an enhanced chemiluminescence (ECL) kit (Thermo scientific, Rockford, IL). Image capture was performed using Chemi-Doc (Bio-Rad).
HaCaT cells were seeded onto glass coverslips and incubated with TI in the absence or presence of CSYJE (500 μg/mL) for 30 min. The cells were fixed in 4 % paraformaldehyde and 100 % acetone, blocked in 0.5 % bovine serum albumin, and incubated with anti-STAT1 antibody (Cell Signaling, Danvers, MA) for 1 h at room temperature. Then, fluorescein isothiocyanate (FITC)-conjugated anti-rabbit immunoglobulin G (IgG) antibody (Invitrogen, Carlsbad, CA) was used as a secondary antibody. The immunostained cells were mounted with medium containing 4′6-diamidino-2-phenylindole (DAPI) and visualized using an Olympus FLUOVIEW FV10i confocal microscope (Tokyo, Japan).
The data are expressed as the mean ± SEM. Data were analyzed using one-way analysis of variance and Dunnett’s multiple comparisons test. Results with a P value < 0.05 were considered to be statistically significant.
Identification and quantification of the marker components in CSYJE
Effects of CSYJE on cell viability of RAW 264.7 mouse macrophages and HaCaT human keratinocytes
CSYJE inhibits production of pro-inflammatory mediators in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells
CSYJE inhibits chemokine production in TNF-α and IFN-γ-stimulated HaCaT cells
CSYJE inhibits STAT1 phosphorylation in TNF-α and IFN-γ-stimulated HaCaT cells
CSYJE, a traditional herbal fomula, is composed of 9 medicinal herbs such as Nelumbinis Semen, Poria Sclerotium, Ginseng Radix, Astragali Radix, Scutellariae Radix, Plantaginis Semen, Liriope Tuber, Lycii Radicis Cortex, and Glycyrrhizae Radix et Rhizoma and has been used to treat neuropsychiatric disease, stroke, renal tuberculosis, stomatitis, pyelitis, and cystitis in Korea . The major components of the 9 herbal medicines are known as follows: flavonoids (e.g. isoquercitrin and quercetin-3-O-glucuronide) from Nelumbinis Semen , triterpenoids (e.g. pachymic acid and dehydropachymic acid) from Poria Sclerotium [19, 20], triterpenoids (e.g. ginsenoside Rb1 and Rb1) from Ginseng Radix , flavonoids (e.g. formononetin and calycosin-7-O-glucoside) from Astragali Radix , flavonoids (e.g. baicalin and wogonoside) from Scutellariae Radix , iridoids (e.g. genoiposide and genipsidic acid) form Plantaginis Semen [24, 25], steroid saponins (e.g. spicatoside A and B) form Liriope Tuber , phenylpropanoid (chlorogenic acid) and flavonoid (e.g. rutin) form Liriope Tuber , and triterpene saponin (e.g. glycyrrhizin) and flavonoids (e.g. liquiritin and liquiritigenin), from Glycyrrhizae Radix et Rhizoma . Among those constituents, we tried to analyze 19 compounds, such as hyperoside, isoquercitrin, pachymic acid, ginsenoside Rb1, ginsenoside Rg1, formononetin, baicalin, baicalenin, wogonoside, wogonin, geniposide, geniposidic acid, spicatoside A, chlorogenic acid, rutin, liquiritigenin, liquiritin, liquiritin apioside, and glycyrrhizin using optimized HPLC–PDA method in CSYJE sample. Consequently, the main components of Scutellariae Radix Glycyrrhizae Radix et Rhizoma, liquiritin apioside, liquiritin, baicalin, wogonoside, and glycyrrhizin were detected. However, other components were not detected in this sample. Finally, baicalin (22.52 ± 0.36 mg/g), marker compound of Scutellariae Radix, was detected as the major compound in CSYJE extract.
In the present study, we found that CSYJE inhibited the actions of various inflammatory mediators. CSYJE inhibited production of the pro-inflammatory mediators NO and PGE2, and cytokine IL-6 in LPS-stimulated macrophages. CSYJE also inhibited the production of the chemokines TARC, MDC, RANTES, and IL-8, and suppressed STAT1 activation in TI-treated keratinocytes.
Macrophages, as innate immune cells, initiate inflammation and the immune response . When challenged with LPS, macrophages are activated and release various pro-inflammatory factors, excessive release of which can result in extensive tissue damage and pathological changes. NO, an important cellular signaling molecule and pro-inflammatory mediator produced by inducible nitric oxide synthase (iNOS), plays a key role in the pathogenesis of inflammation caused by its overproduction in abnormal situations . PGE2 is another important pro-inflammatory factor regulated by cyclooxygenase-2 (COX-2). Its expression is elevated in LPS-stimulated macrophages, and is associated with many chronic inflammatory diseases including cardiovascular diseases, arthritis, inflammatory bowel disease, angiogenesis, and chronic gastric ulcers [31–33]. Macrophages also release pro-inflammatory cytokines including IL-1β, IL-6, and TNF-α, which are used to evaluate potential anti-inflammatory properties of agents against LPS-induced macrophage activation . In the present study, CSYJE significantly inhibited the concentrations of NO and PGE2 in the supernatant in LPS-stimulated RAW 264.7 cells. Thus, CSYJE might reduce inflammatory responses by inhibiting IL-6 secretion.
Keratinocytes produce various chemokines, which are involved in the development of inflammatory diseases. TARC and MDC are produced by dendritic cells, endothelial cell and keratinocytes which bind and attract CCR4+ Th2 cells into inflammatory tissues . Several studies have reported that there are high levels of TARC and MDC in the serum of patients with atopic dermatitis . The severity of atopic dermatitis was significantly correlated with these chemokine levels . RANTES is overexpressed in the keratinocytes of such patients. In addition, RANTES has a significant role in the inflammatory process of psoriasis. Here, we found that CSYJE inhibited TARC, MDC, RANTES, and IL-8 production in TI-stimulated HaCaT keratinocytes.
STAT1 is also a pivotal regulator of IFN-γ-induced immune responses . The activated STAT1 pathway can modulate the expression of numerous inflammatory mediators, including chemokines . Here we demonstrated that CSYJE inhibited the TI-induced phosphorylation and nuclear translocation of STAT1 in HaCaT cells. These data suggest that CSYJE might block the induction of chemokine production including TARC, MDC, and RANTES in TI-treated keratinocytes by inhibiting STAT1 activation.
Our present study demonstrated that CSYJE inhibited the levels of NO, PGE2, and IL-6 in LPS-stimulated RAW 264.7 cells, and reduced the production of chemokines TARC, MDC, RANTES, and IL-8 by suppressing the activation of STAT1 in TI-stimulated HaCaT cells. Further elucidation of the signaling pathways involved in the inhibition of inflammatory mediators by CSYJE is now needed to facilitate the design of therapeutic agents for inflammatory diseases.
This research was supported by a grant for “Construction of Scientific Evidence for Herbal Medicine Formulas (K14030)”, from the Korea Institute of Oriental Medicine (KIOM).
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