- Research article
- Open Access
- Open Peer Review
Antioxidant and in vitro anticancer activities of phenolics isolated from sugar beet molasses
© Chen et al. 2015
- Received: 19 May 2015
- Accepted: 2 September 2015
- Published: 7 September 2015
In the present study, the phenolic compounds were prepared using ultrasonic-aid extraction from sugar beet molasses (SBM).
Gallic acid (GA), cyanidin-3-O-glucoside chloride (CGC) and epicatechin (EP) were produced after column chromatography from the extraction, and further detected using NMR, QTOF-MS and ESI-MS/MS.
The three compounds exhibited strong antioxidant activities including DPPH radical scavenging activities, ABTS radical scavenging activities and ORAC values. GA showed the strongest antioxidant activity. Antitumor activities significantly increased in a dose-dependent manner. In particular, the CGC had growth inhibitory activities of 94.86, 87.27 and 67.13 % against the human colon (CACO-2), hepatocellular (HepG2) and breast (MCF-7) carcinoma cell lines, respectively, at the highest concentration of 400 μg/mL of the extracts. These results suggest that the three compounds are key chemical compositions valuable for preparing functional foods in the food industry.
The results suggested that SBM is a natural source of antioxidant and antitumor agents for preparing functional foods.
- Sugar beet molasses
- Phenolic compounds
- Antioxidant activities
- Antitumor activities
Sugar beet is an important sugar crop cultivated for sugar production [1, 2]. Sugar beet molasses (SBM) is a by-product in sugar beet processing . Early studies found that SBM can be used to produce alcohol and fermentation medium [4, 5]. While recent study reported that SBM showed higher antioxidant activity, anti-inflammatory and anti-proliferative activities [6, 7], the bioactive components of SBM are mainly phenolics, alkaloids, tannins, saponins, terpenoids, steroids, and volatile oil , and specific bioactive compounds such as syringic acid, vanillin, ferulic acid, hydroxybenzaldehyde, hydroxybenzoic acid, luteolin/kaempferol, feruloyl-arabinose-arabinose and caffeoyltartaric acid which have been prepared and identified .
Phenolic contents have been demonstrated to have a variety of bioactivities such as anti-aging, anti-fatigue, anti-hypoxia, immunological, anti-radiation, anti-inflammatory, anti-proliferative and hypoglycemic effects [10–14]. Meanwhile many investigations revealed that phenolic contents contribute to the antioxidant and antitumor activities of plants . Antioxidants can restrict the deleterious effects of these oxidant reactions and these restrictions can involve scavenging free radicals or preventing radical formation . However, to the best of our knowledge, there were few reports on the antioxidant and antitumor activities of phenolics from sugar beet molasses (SBMP). Given the rich natural resources of SBM, the extraction researches of SBMP will have a highly practical value.
The primary objective of the present study is to separate and purify the phenolic compounds in SBM, identify the structure of prepared phenolic compounds, and study the bioactivity of the prepared phenolic compounds in SBM.
Materials and reagents
SBM was provided by Xinjiang Green Xiang Sugar Industry Co., Ltd (Tacheng, China). 2,2-diphenyl-1-picryhydrazyl (DPPH), 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), ascorbic acid (Vc), fluorescein, 2,2′-azobis (2-methylpropionamidine) dihydrochloride (AAPH), trolox [(±)-6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid] and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Dulbecco’s modified eagle medium (DMEM), penicillin-streptomycin were purchased from Gibco Co. (Long Shang Industry Park, Beijing, China). Fetal bovine serum (FBS) was purchased from Zhejiang Tianhang Biological Technology Co. (Zhejiang, China). Methanol (HPLC-grade) was purchased from Merck Co. (Whitehouse Station, NJ, USA). Ultra-pure water was prepared by a Milli-Q system (Millipore, Bedford, USA).
Extraction, isolation and purification
The SBM (2.0 kg) was extracted by 30 mL 70 % (w/v) ethanol using a RK102H ultrasonic (BANDELIN SONOREX, Germany). Extraction conditions were ultrasonic power, 450 W, HCl concentrations 1.6 mol/L, temperature 40 °C and time 60 min. The extract was concentrated at 45 °C in vacuum using a rotary evaporator (RE-52 A, Yarong Co. Ltd., Shanghai, P. R. China) to obtain the total extraction fraction (634.02 g). Then, the total extracted fraction was suspended in distilled water. The resulting solution was successively partitioned with different solvents which yielded petroleum ether fraction, chloroform fraction, ethyl acetate fraction, n-butanol fraction and aqueous fraction. After drying in vacuo, five fractions were obtained. These fractions included the petroleum ether fraction (5.26 g, yield coefficient 0.83 %), chloroform fraction (32.97 g, yield coefficient 5.20 %), ethyl acetate fraction (76.7 g, yield coefficient 12.10 %), n-butanol fraction (58.71 g, yield coefficient 9.26 %), and water fraction (142 g, yield coefficient 22.40 %). In the biological activity screening tests, the ethyl acetate fraction showed stronger antioxidant and antitumor activities than other four fractions. Therefore, the ethyl acetate fraction was chosen for further purification.
Identification of purity
Sample purity was analyzed using an HPLC-DAD system, which consisted of a C18 column (XBridge™ Shield, RP18, 5 μm, 250 mm × 4.6 mm), a Waters 600 pump and a Waters 2998 DAD (Waters, Milford, USA), column temperature was set at 35 °C. The mobile phase was 1 % acetic acid aqueous solution (A) and methanol (B) with a gradient program of 0–5 min, linear gradient 5–10 % B; 5–35 min, linear gradient 10–20 % B; 35–45 min, 20 % B isocratic; 45–65 min, linear gradient 20–40 % B; 65–70 min, linear gradient 40–100 % B and 70–90 min 100 % B isocratic elution at a flow rate of 1 mL/min. The injection volume is 20 μL, and the detection wavelengths were set at 280 and 360 nm.
Identification of compounds
The purified compounds from SBM were identified by electrospray ionization-mass spectrometry (ESI-MS), 1H NMR and 13C NMR spectrometry. The ESI-MS was carried out in a LCQ-Fleet mass spectrometer (Thermo Fisher Scientific, Waltham, MA), with an electrospray ionization source using a negative mode (m/z 50–800). 1H NMR spectra and 13C NMR spectra were recorded on a Bruker Avance DMX-500 spectrometer (Bruker Biospin GmbH, Germany), operating at 500 and 125 MHz for 1H and 13C, respectively, using D2O or deuterated dimethyl sulfoxide (DMSO-d6). In D2O, tetramethylsilane (TMS) was used as the internal standard. In DMSO-d 6 , the residual solvent was used as the internal standard. Chemical shifts were expressed in δ (ppm) downfield from TMS as an internal standard, and coupling constants were reported in hertz.
Assay of total antioxidant activity
The oxygen radical absorbance capacity (ORAC) assay was carried out according to the modified method of Ou et al. . Briefly, sample solution was prepared with 75 mM phosphate buffer (pH 7.4). The 20 μL sample aliquots or Trolox standard (6.25–100 μM) were added in a black 96-well plates (Greiner Bio-one Cellstar, Frickenhausen), and then 200 μL of 95.6 nM fluorescein solution was added to each well. The mixture was incubated for 30 min at 37 °C. Finally, 20 μL of AAPH was automatically injected, and the microplate was shaken for 20 s before each measurement. The fluorescence was measured using a Varioskan Flash Multimode Reader (Thermo Fisher Scientific Inc.) at excitation of 540 nm and emission of 565 nm for 35 cycles every 3.0 min. The buffer was used as blank. The ORAC value (μmol Trolox equivalents (TE)/g) refers to the required Trolox content (μmol) when ORAC of Trolox is equal to 1 g sample.
Determination of antitumor activity
Human colon (CACO-2), hepatocellular (HepG2) and breast (MCF-7) carcinoma cell lines were provided by the Medical College of Sun Yat-Sen University (Guangzhou, China). The cells were cultured in DMEM containing 10 % FBS, 100 μg/mL streptomycin and 100 U/mL penicillin in a humidified incubator with 5 % CO2 at 37 °C.
Data were analyzed using SPSS (SPSS Inc., Chicago, IL, USA) and presented as mean ± SD with triplicates. Significance was determined at p < 0.05 by analysis of variance (ANOVA) followed by Duncan’s least significant test.
Three compounds isolated and obtained from SBM were identified as gallic acid (GA), cyanidin-3-O-glucoside chloride (CGC) and epicatechin (EP). Their spectroscopic data were listed below.
Gallic acid (Compound 1): C7H6O5, white powder, purity, 99.2 %. QTOF-MS m/z: 177.0158 [M + Na-H2O]+, ESI-MS/MS m/z: 177, 153, 127. 1H-NMR (500 MHz, CDCl3) δ H: 7.12 (2H, s, H-2, 6). 13C-NMR (125 MHz, CDCl3) δ C: 121.2 (C-1), 109.4 (C-2), 145.3 (C-3), 138.6 (C-4), 145.3 (C-5), 109.4 (C-6), 168.5 (COOH) .
Cyanidin-3-O-glucoside chloride (Compound 2): C21H21O11, dark brown crystalline powder, purity, 98.7 %. QTOF-MS m/z:450.1157 [M + H]+, ESI-MS/MS m/z:450, 287. 1H-NMR (CD3COCD3, 500 MHz) δ H: 8.92 (1H, s, H-4), 6.88 (1H, d, J = 2.0 Hz, H-6), 6.91 (1H, s, H-8), 8.10 (1H, d, J = 1.0 Hz, H-2′), 7.02 (1H, d, J = 8.5Hz, H-5′), 8.26 (1H, d, J = 7.5Hz, H-6′), 5.28 (1H, d, J = 4.5Hz, H-1″), 3.13 (1H, t, H-4″), 3.38 (1H, q, H-3″), 3.31 (1H, q, H-2″), 3.48 (1H, m, H-5″), 3.82 (1H, m, H-6a″), 3.71 (1H, m, H-6b″) .
Epicatechin (Compound 3): C15H14O6, white crystalline powder, purity, 99.5 %. QTOF-MS m/z:291.0863 [M + H]+, ESI-MS/MS m/z: 291, 273, 165, 139. 1H-NMR (CD3COCD3, 400 MHz) δ H: 7.03 (1H, d, J = 2.0 Hz, H-2′), 6.82 (1H, d, J = 8.0 Hz, H-5′), 6.77 (1H, dd, J = 8.0, 2.0 Hz, H-6′), 5.99 (1H, d, J = 2.0 Hz, H-8), 5.89 (1H, d, J = 2.0 Hz, H-6), 4.86 (1H, s, H-2), 4.11 (1H, m, H-3), 2.85 (1H, dd, J = 16.4, 4.4 Hz, H-4), 2.72 (1H, dd, J = 16.4, 3.2 Hz, H-4). 13C-NMR(CD3COCD3, 100 MHZ,) δ C: 28.83 (C-4), 68.33 (C-3), 82.70 (C-2), 95.41 (C-8), 96.13 (C-6), 100.62 (C-10), 115.23 (C-5′), 115.70 (C-2′), 120.06 (C-6′), 132.14 (C-1′), 145.64 (C-3′), 145.72 (C-4′), 156.89 (C-9), 157.22 (C-5), 157.73 (C-7) [24, 25].
According to the results of DPPH, ABTS radical scavenging and ORAC assays, the ethyl acetate fraction showed the strongest antioxidant activity among five extraction fractions. The three compounds isolated from the ethyl acetate fraction could be explored as natural antioxidants as they showed strong antioxidant activities. GA showed the strongest antioxidant activity, followed by Vc which is greater than those of the CGC while EP showed the least antioxidant activity.
The three compounds, GA, CGC and EP, obtained in our study are three typical phenolic compounds in plants which have been reported to possess strong antioxidant activity [26, 27]. According to reports, free hydroxyl groups in phenolics are mainly responsible for antioxidant activity [28, 29]. GA, CGC and EP, belonging to the phenolic compounds, are rich in multiple phenolic hydroxyl groups, which have been considered to be important antioxidants for a long time, and their antioxidant activities could be attributed to the numerous hydroxyl groups present on their structures. However, according to the literature [29, 30], the number of hydroxyl groups bonded to the aromatic ring and their positions are probably the most important but not the only factors influencing the antioxidant activities of phenolic components. Meanwhile, character of substituents (carboxyl or acetyl group) and their position in relation to the hydroxyl groups seem to influence the antioxidant or anti-radical features.
As shown in Fig. 6, GA, CGC and EP showed significant antitumor activities. CGC exhibited the strongest inhibitory effect on CACO-2 (Fig. 6a), HepG2 (Fig. 6b) and MCF-7 (Fig. 6c) cells. The inhibitory rate of CGC (400 μg/mL) was about 95 %. Additionally, the inhibitory rates of CGC (all concentrations) were much higher than that of 5-fluorouracil. However, GA and EP showed relatively weak inhibitory effects on the CACO-2, HepG2 and MCF-7 cells, and the inhibitory rates increased slowly with the increase in their concentration. From previous studies, compounds with higher antioxidant activities always had higher antitumor activity and prevented the cellular senescence and apoptosis [9, 31]. However, although CGC showed lower antioxidant activity than GA, it had the strongest inhibitory effects on the cancer cell proliferation.
Early studies showed that GA, CGC and EP had anticancer activity. GA has been shown to have antitumor activity in many cancer cells without damaging normal cells [32–36]. Wang et al.  reported GA induced apoptosis by triggering the extrinsic or Fas/FasL pathway as well as the intrinsic or mitochondrial pathway in MCF-7 cells. Filipiak et al.  reported CGC showed inhibitory activity against gelatinases corresponding to its cytotoxic activity in HT1080 cells. Xu et al.  reported CGC attenuated ethanol-induced migration/invasion of breast cancer cells expressing high levels of ErbB2 (BT474, MDA-MB231 and MCF7ErbB2) in a concentration dependent manner. Zhao et al.  reported GA significantly decreased human cervical cancer cell proliferation and tube formation in human umbilical vein endothelial cells. Siddique et al.  reported EP could cause a decrease in the proliferation, guanosine triphosphate-bound Ras protein, Akt phosphorylation and NF-κB transcriptional activity of premalignant and malignant Krasactivated PDE cells. Though the antitumor activities of these compounds had been previously reported, the mechanism(s) of antitumor activity of the individual compound needs to be investigated. However, the presence of the three compounds may be responsible for the antioxidant and antitumor activities exhibited by SBM and suggested that SBM might be used as an additive in antitumor food.
Three compounds, Gallic acid (GA), cyanidin-3-O-glucoside chloride (CGC) and epicatechin (EP) and the main compounds in SBM with high antioxidant and antitumor activities. GA possessed the strongest antioxidant activity. CGC showed strong antitumor activities against human colon (CACO-2), hepatocellular (HepG2) and breast carcinoma cells. The antitumor activity was higher than positive control (5-fluorouracil). Further studies will be needed to investigate other chemical compounds and bioactivity of SBM. These results suggest that SBM is a potential source of antioxidant and antitumor agents for preparing functional foods.
All the authors declare that there are no conflicts of interest. All authors acknowledge the ministry of Science and Technology in Agriculture Science and Technology Achievements Transformation Fund Project (No.2013GB23600669), the Science and Technology Planning Project of Guangzhou Municiple, China (No. 2011Y2-00012), China Postdoctoral Science Foundation funded project (2014 M560664) and this work was supported by the basic research foundation of SCUT (2012ZM0072).
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- McDill BM. Beet Sugar Industry. Ind Eng Chem. 1947;39(5):657–69.Google Scholar
- Balbach JK. The effect of ownership on contract structure, costs, and quality: the case of the U.S. beet sugar industry. In: Royer JS, Rogers RT (eds). The industrialization of agriculture: vertical coordination in the U.S. food system. Aldershot, United Kingdom: Ashgate Publishing Ltd.; 1998. p. 155–184.Google Scholar
- Roukas T. Ethanol production from non-sterilized beet molasses by free and immobilized < i > Saccharomyces cerevisiae</i > cells using fed-batch culture. J Food Eng. 1996;27(1):87–96.View ArticleGoogle Scholar
- Ahmedna M, Marshall W, Rao R. Production of granular activated carbons from select agricultural by-products and evaluation of their physical, chemical and adsorption properties. Bioresour Technol. 2000;71(2):113–23.View ArticleGoogle Scholar
- Paturau JM. By-products of the cane sugar industry. An introduction to their industrial utilization. Amsterdam: Elsevier Science Publishers BV; 1989.Google Scholar
- Filipčev B, Lević L, Bodroža-Solarov M, Mišljenović N, Koprivica G. Quality characteristics and antioxidant properties of breads supplemented with sugar beet molasses-based ingredients. Int J Food Prop. 2010;13(5):1035–53.View ArticleGoogle Scholar
- Koprivica G, Mišljenović N, Lević L, Kuljanin T. Influence of the nutrients present in sugar beet molasses and saccharose solutions on the quality of osmodehydrated carrot. Časopis za procesnu tehniku i energetiku u poljoprivredi/PTEP. 2009;13(2):184–7.Google Scholar
- Paananen H, Kuisma J. Chromatographic separation of molasses components. Zuckerindustrie. 2000;125(12):978–81.Google Scholar
- Valli V, Gómez-Caravaca AM, Di Nunzio M, Danesi F, Caboni MF, Bordoni A. Sugar cane and sugar beet molasses, antioxidant-rich alternatives to refined sugar. J Agric Food Chem. 2012;60(51):12508–15.View ArticlePubMedGoogle Scholar
- Khadem S, Marles RJ. Monocyclic phenolic acids; Hydroxy- and polyhydroxybenzoic acids: Occurrence and recent bioactivity studies. Molecules. 2010;15(11):7985–8005.View ArticlePubMedGoogle Scholar
- Liu X, Zhao M, Wu K, Chai X, Yu H, Tao Z, et al. Immunomodulatory and anticancer activities of phenolics from emblica fruit (Phyllantlius emblica L.). Food Chem. 2012;131(2):685–90.View ArticleGoogle Scholar
- Pereira DM, Valentao P, Pereira JA, Andrade PB. Phenolics: From chemistry to biology. Molecules. 2009;14(6):2202–11.View ArticleGoogle Scholar
- Vineetha VP, Girija S, Soumya RS, Raghu KG. Polyphenol-rich apple (Malus domestica L.) peel extract attenuates arsenic trioxide induced cardiotoxicity in H9c2 cells via its antioxidant activity. Food Funct. 2014;5(3):502–11.View ArticlePubMedGoogle Scholar
- Wu L-C, Jou AF-J, Chen S-H, Tien C-Y, Cheng C-F, Fan N-C, et al. Antioxidant, anti-inflammatory and anti-browning activities of hot water extracts of oriental herbal teas. Food Funct. 2010;1(2):200–8.View ArticlePubMedGoogle Scholar
- Ravipati AS, Zhang L, Koyyalamudi SR, Jeong SC, Reddy N, Bartlett J, et al. Antioxidant and anti-inflammatory activities of selected Chinese medicinal plants and their relation with antioxidant content. BMC Complement Altern Med. 2012;12:173.Google Scholar
- Gomaa EZ. In vitro antioxidant, antimicrobial, and antitumor activities of bitter almond and sweet apricot (Prunus armeniaca L.) kernels. Food Sci Biotechnol. 2013;22(2):455–63.View ArticleGoogle Scholar
- Luo A, He X, Zhou S, Fan Y, Luo A, Chun Z. Purification, composition analysis and antioxidant activity of the polysaccharides from < i > Dendrobium nobile</i > Lindl. Carbohydr Polym. 2010;79(4):1014–9.View ArticleGoogle Scholar
- Thaipong K, Boonprakob U, Crosby K, Cisneros-Zevallos L, Hawkins Byrne D. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. J Food Compos Anal. 2006;19(6):669–75.View ArticleGoogle Scholar
- Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 1999;26(9):1231–7.View ArticlePubMedGoogle Scholar
- Ou B, Huang D, Hampsch-Woodill M, Flanagan JA, Deemer EK. Analysis of antioxidant activities of common vegetables employing oxygen radical absorbance capacity (ORAC) and ferric reducing antioxidant power (FRAP) assays: a comparative study. J Agric Food Chem. 2002;50(11):3122–8.View ArticlePubMedGoogle Scholar
- Zhou J-J, Yue X-F, Han J-X, Yang W-Y. Improved MTT assay for activity of antitumor agents. Chin J Pharm. 1993;24:455–7.Google Scholar
- Werner I, Bacher A, Eisenreich W. Retrobiosynthetic NMR studies with C-13-labeled glucose - Formation of gallic acid in plants and fungi. J Biol Chem. 1997;272(41):25474–82.View ArticlePubMedGoogle Scholar
- Fernandes A, Ivanova G, Bras NF, Mateus N, Ramos MJ, Rangel M, et al. Structural characterization of inclusion complexes between cyanidin-3-0-glucoside and beta-cyclodextrin. Carbohydr Polym. 2014;102:269–77.View ArticlePubMedGoogle Scholar
- Cren-Olive C, Wieruszeski JM, Maes E, Rolando C. Catechin and epicatechin deprotonation followed by C-13 NMR. Tetrahedron Lett. 2002;43(25):4545–9.View ArticleGoogle Scholar
- Berregi I, Santos JI, del Campo G, Miranda JI. Quantitative determination of (−)-epicatechin in cider apple juices by H-1 NMR. Talanta. 2003;61(2):139–45.View ArticlePubMedGoogle Scholar
- Panyathep A, Chewonarin T, Taneyhill K, Vinitketkumnuen U. Antioxidant and anti-matrix metalloproteinases activities of dried longan (Euphoria longana) seed extract. Scienceasia. 2013;39(1):12–8.View ArticleGoogle Scholar
- Othman A, Jalil AMM, Weng KK, Ismail A, Abd Ghani N, Adenan I. Epicatechin content and antioxidant capacity of cocoa beans from four different countries. Afr J Biotechnol. 2010;9(7):1052–9.View ArticleGoogle Scholar
- Feng J-Y, Liu Z-Q. Phenolic and enolic hydroxyl groups in curcumin: Which plays the major role in scavenging radicals? J Agric Food Chem. 2009;57(22):11041–6.View ArticlePubMedGoogle Scholar
- Sroka Z, Cisowski W. Hydrogen peroxide scavenging, antioxidant and anti-radical activity of some phenolic acids. Food Chem Toxicol. 2003;41(6):753–8.View ArticlePubMedGoogle Scholar
- Vulic JJ, Cebovic TN, Canadanovic VM, Cetkovic GS, Djilas SM, Canadanovic-Brunet JM, et al. Antiradical, antimicrobial and cytotoxic activities of commercial beetroot pomace. Food Funct. 2013;4(5):713–21.View ArticlePubMedGoogle Scholar
- Valko M, Leibfritz D, Moncol J, Cronin MTD, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol. 2007;39(1):44–84.View ArticlePubMedGoogle Scholar
- Wang K, Zhu X, Zhang K, Zhu L, Zhou F. Investigation of gallic acid induced anticancer effect in human breast carcinoma MCF-7 cells. J Biochem Mol Toxicol. 2014;28(9):387–93.View ArticlePubMedGoogle Scholar
- Filipiak K, Hidalgo M, Manuel Silvan J, Fabre B, Carbajo RJ, Pineda-Lucena A, et al. Dietary gallic acid and anthocyanin cytotoxicity on human fibrosarcoma HT1080 cells. A study on the mode of action. Food Funct. 2014;5(2):381–9.View ArticlePubMedGoogle Scholar
- Xu M, Bower KA, Wang SY, Frank JA, Chen G, Ding M, et al. Cyanidin-3-Glucoside inhibits ethanol-induced invasion of breast cancer cells overexpressing ErbB2. Mol Cancer. 2010;9:285.View ArticlePubMedPubMed CentralGoogle Scholar
- Zhao B, Hu M. Gallic acid reduces cell viability, proliferation, invasion and angiogenesis in human cervical cancer cells. Oncology Letters. 2013;6(6):1749–55.PubMedPubMed CentralGoogle Scholar
- Siddique HR, Liao DJ, Mishra SK, Schuster T, Wang L, Matter B, et al. Epicatechin-rich cocoa polyphenol inhibits Kras-activated pancreatic ductal carcinoma cell growth in vitro and in a mouse model. Int J Cancer. 2012;131(7):1720–31.View ArticlePubMedGoogle Scholar