Alternative and antioxidant therapies used by a sample of infertile males in Jordan: a cross-sectional survey
© Bardaweel; licensee BioMed Central Ltd. 2014
Received: 13 January 2014
Accepted: 30 June 2014
Published: 16 July 2014
Complementary and alternative medicine (CAM) is frequently used in the Middle East, especially to treat chronic diseases such as infertility. We aimed to examine the prevalence, characteristics, and determinants of CAM use, particularly herbs and antioxidant therapies, among infertile males presenting for infertility evaluation in Jordan.
Demographic information, use of alternative and antioxidant therapies for infertility treatment, and patients’ belief in efficacy and safety of the therapies used were collected using a face-to-face questionnaire. Data were collected from 428 infertile male patients presenting at infertility clinics in Amman, the capital city of Jordan. The study was conducted between April 2013 and September 2013.
Of the 428 men who completed the questionnaire, 184 (43%) used at least one of the alternative and antioxidant therapies specified in the questionnaire. Nutritional regime; vitamins, such as vitamins C and E; and medicinal herbs, such as ginger, saw palmetto, and ginseng were the most commonly used therapies reported. A correlation between the use of alternative and antioxidant therapies versus infertility duration was found. Additionally, the majority of males using CAM did not inform their health care providers about their usage.
The high prevalence of CAM use among infertile male patients underscores the urge to assimilate CAM into the education and training of health professionals, as well as to improve infertile patients’ knowledge of the safe use of CAM modalities.
KeywordsCAM Male infertility Antioxidants Medicinal plants
Infertility affects approximately 10–15% of all couples in industrialized countries [1–3]. Approximately 30% to 50% of these cases are owing to male factor infertility . Male factor infertility is defined as a change in sperm concentration, motility, and/or morphology in at least one sample of two sperm analyses collected between 1 and 4 weeks .
The generation of oxidants, also known as reactive oxygen species (ROS), in the male reproductive tract is a concern because of their potential toxic effects at high levels on sperm quality and function [5, 6]. The oxygen paradox is a challenge faced by all cells living under aerobic conditions, including spermatozoa. While oxygen is required for life, its ROS metabolites endanger cell survival and functions . Male germ cells, at various differentiation stages, generate minute amounts of ROS that are required for sperm production and maturation [8, 9]. To preserve normal cell function, excess ROS must be constantly eliminated by seminal plasma antioxidants. Excessive ROS generation may adversely affect sperm number, motility, quality, and function including damage to sperm DNA integrity [10–12]. Several studies have demonstrated that elevated ROS levels are detected in the semen of 25% to 40% of infertile men [7, 12, 13]. Spermatozoa are intensely vulnerable to the damage provoked by excessive ROS as their plasma membranes enclose considerable amounts of polyunsaturated fatty acids  while their cytoplasm has low concentrations of ROS-scavenging enzymes .
Enzymatic antioxidants, such as superoxide dismutase, the glutathione peroxidase/glutathione reductase system, and catalase , as well as non-enzymatic antioxidants, such as ascorbate (vitamin C) , tocopherol (vitamin E) , taurine, and hypotaurine , are available in the seminal plasma to prevent critical damage provoked by ROS. In fact, the seminal plasma of fertile men has a higher total antioxidant capacity than the seminal plasma of infertile men . However, elevated levels of ROS measured in the semen of infertile men are more likely owing to increased ROS production rather than to reduced antioxidant capacity of the seminal plasma .
While the available treatments for male infertility have erratic success rates [21, 22], assisted reproductive technologies, such as intrauterine insemination and in vitro fertilization, potentially enhance the ability of infertile men to contribute to a pregnancy. For many infertile men, the lack of accessible successful medical therapies has led to the use of alternative therapies, including the use of herbs and antioxidants. The use of alternative therapies in infertile men is usually associated with speculation that some forms of male infertility are caused by antioxidant deficiency. Therefore, the hypothesis is that supplementary antioxidants may enhance their ability to contribute to a pregnancy.
We aimed to gain insights into the patterns of use of alternative and antioxidant therapies in infertile men in Jordan. The prevalence and factors leading to the use of such therapies among infertile men were also investigated. Moreover, one of the objectives of the study was to find out which complementary and alternative medicines (CAM) are being used by infertile men, and whether they believed in the effectiveness of these therapies.
Verbal informed consent to participate in the study was obtained based on a standard written statement. Ethical approval for conducting the study was obtained from the Institutional Review Board (IRB) at the Jordan University Hospital (JUH) and the Scientific Committee at the Deanship of Scientific Research at The University of Jordan. Patient information for this study remained confidential and within the institution.
Data were collected from male patients seeking infertility treatment in two types of facilities: in vitro fertilization (IVF) centers, at both public and private hospitals, and infertility private clinics. In the private clinics, patients were seen for routine examination and treatment of infertility without any conception assistance being offered. Patients at IVF centers were given conception assistance. Both types of facilities were distributed in different areas of Amman, the capital city of Jordan. The study took place between April 2013 and September 2013. Only male patients attending any of the study centers were invited to complete the questionnaire.
The study questionnaire was structured based on preliminary discussions with patients and health professionals. Additionally, the questionnaire was validated by a committee whose members were health professionals, consisting of two fertility specialists, a pharmacist, and a nutritionist. The questionnaire was written in English and then translated into Arabic. Both versions of the questionnaire were checked by three members of the public with no medical background.
The questionnaire was composed of 18 questions and divided into three sections (Additional file 1). The first section considered patients’ demographic information that may have an impact on their health-related attitudes. In particular, patients were asked about age, education, and income level. In the second section, patients were asked about their medical and infertility history. The third section involved questions about the use of antioxidant therapies, as well as other forms of alternative therapies for their infertility condition. Additionally, patients were asked whether they informed their principal healthcare provider about usage of the reported alternative and antioxidant therapies.
The 18-point anonymous structured questionnaire was given to 428 infertile males presenting at any of the study centers. The study population was then subgrouped into two groups, Group A and Group B. Group A included patients who reported the use of alternative and antioxidant therapies as an infertility treatment aid, and Group B included patients who did not use any form of alternative and antioxidant therapies for their infertility treatment.
Data were coded, entered, and analyzed using the Statistical Package for Social Sciences program (SPSS) database for Windows, version 17 (SAS Institute, Cary, NC, USA). The analysis of answers involved descriptive quantitative statistics, like frequency and percentage. Chi-square and Fisher exact tests were used to test for significant association between groups. All hypothesis testing was two-sided, with a probability value of 0.05 deemed significant.
Characteristics of infertile males participating in the study at Amman, Jordan 2013 (N = 428)
Age (N = 428)
Education (N = 428)
Income JD a (N = 418) b
Less than 500 JD
More than 2000 JD
Occupation (N = 414) b
Employee in medical sector
Employee in non medical sector
Infertility period (N = 428)
More than 4 years
Likewise, statistical analysis of the response to a question asking whether participants discussed their CAM use, including their diet, with their physicians, revealed that only 10.3% (19/184) of Group A participants declared their CAM use to their health care provider. Surprisingly, these participants asserted that their health care providers neither approved nor denied their alternative and antioxidant therapy use. Participants indicated that their health care providers were neutral towards their CAM use.
Male infertility is a multi-faceted condition with several potential contributing factors. Most commonly, occupational, environmental, and nutritional factors contribute to sperm count and motility. Sperm count and motility are ultimately responsible for fertility. Spermatogenesis is an energetic process that demands an optimal intake of antioxidants, minerals, and nutrients. Disruption of the balance between these nutrients is suggested as one of the possible etiologies of male infertility .
ROS, such as the superoxide anion, hydrogen peroxide, and nitric oxide, are needed for fertilization, and thus are produced by spermatozoa. Nevertheless, high concentrations of these free radicals can directly damage sperm cells , resulting in male infertility. Antioxidants are the first line of defense against ROS-induced male infertility. Numerous clinical trials have investigated the potential role of antioxidant therapy to relieve ROS-induced male factor infertility . Although antioxidants are hypothesized to be useful therapeutic agents for male infertility, the results of clinical trials are controversial [23, 24]. In this study, we investigated the patterns of antioxidant use among infertile male patients in Jordan. Additionally, the use of alternative therapies, including herbal products and nutritional regime, were evaluated in a population of 428 infertile males in Jordan.
The human body employs three general tactics of antioxidants to safeguard against free radicals: endogenous antioxidants, dietary antioxidants, and metal-binding proteins . Low molecular weight molecules, such as coenzyme Q-10, and larger molecular enzymes, such as superoxide dismutase, catalase, and glutathione peroxidase, are classified as endogenous antioxidants . Vitamins C and E, carotenoids, and flavonoids are classified as dietary antioxidants that act through scavenging free radicals to break the chain reaction responsible for lipid peroxidation. This class of antioxidants is known as chain-breaking antioxidants . On the other hand, metal-binding proteins, such as albumin, ferritin, and myoglobin, inactivate the transition metal ions that catalyze the production of free radicals [26, 27].
In this study sample, the use of chain-breaking antioxidants, such as vitamin C and vitamin E, was more frequently encountered relative to other antioxidants (Figure 4). Vitamin C (ascorbic acid) is a water-soluble ROS scavenger, while vitamin E is an important lipid-soluble antioxidant. The hydrophilicity and lipophilicity of vitamins C and E may function synergistically to scavenge ROS and protect spermatozoa. Although no decisive conclusion has been confirmed in regard to the beneficial role of vitamins C and E in male infertility , some reports suggest that sperm motility and characteristics deteriorated significantly when a combination of vitamins C and E was used [23, 28, 29].
Additionally, our study demonstrates that a significant percentage of men presenting for infertility assessment have used herbs as adjunct therapies to enhance their fertility (Figure 4). The potential of herbs to enhance male fertility has not been extensively studied [30, 31]. According to our results, ginger is one of the main herbal ingredients used by Group A participants. Ginger extracts have been widely studied for an extensive scope of biological activities, especially antioxidant activities . In rats, ginger considerably reduced lipid peroxidation by sustaining the activities of antioxidant enzymes superoxide dismutase, catalase, and glutathione peroxides . Saw palmetto was also one of the main herbs used to treat infertility among Group A participants. Recently, the use of saw palmetto for the treatment of benign prostatic hyperplasia and lower urinary tract symptoms in males has increased [33, 34]. Nevertheless, the precise mechanism of action of saw palmetto remains unknown.
Recent studies suggest several beneficial effects of ginseng to increase the antioxidant capacity of various tissues . Both Asian and American ginseng were shown to enhance libido and copulatory performance in laboratory animals [36, 37]. The observed effects of ginseng are possibly related to its ginsenoside components on the central nervous system and gonadal tissues [36, 37]. In addition, ginsenosides were shown to increase sperm motility  and facilitate erectile dysfunction in males .
One of the main points addressed by our analysis is the poor communication between the patients and their health care providers. Only 10.3% (19/184) of Group A participants declared their alternative and antioxidant therapies to their physicians. Increased consumption of certain antioxidants, regardless of their source, with the concurrent use of herbal products rich in antioxidants has been suggested to significantly deteriorate sperm motility and characteristics [28, 29]. Although our study design was unable to establish a negative or positive correlation between the use of alternative and antioxidant therapies and an increase in pregnancy rate, the increased prevalence of alternative and antioxidant therapies among infertile males necessitates a clear and urgent need for physicians to become more aware of this phenomenon and conduct further research in this field .
This study provides results of a survey of infertile males in Jordan (n = 428) seeking medical assistance for their infertility. Our data show that the use of alternative and antioxidant therapies for infertility treatment is more common in middle-aged and fairly educated infertile males who have suffered from infertility for more than 4 years. Nutritional regime, herbal formulations, and vitamins such as vitamin C and E were the most popular alternative and antioxidant therapies used in the study population. Our results indicate that the majority of infertile males who used alternative and antioxidant therapies did not inform their health care providers about their adjunct therapies. Some of these adjunct treatments might have adverse effects on the outcomes of conventional medical treatment. Therefore, it is essential to inquire about the use of these therapies by infertile males presenting at infertility clinics.
The survey that this article is based on was financially supported by The University of Jordan. Data collection was accomplished by a volunteer group of fifth-year pharmacy students. The author would like to thank the volunteers who helped in data collection and SPSS data entry, as well as all physicians at the study centers who encouraged their patients to participate.
- Evers J: Female subfertility. Lancet. 2002, 360: 151-159.View ArticlePubMedGoogle Scholar
- Sharlip ID, Jarow JP, Belker AM, Lipshultz LI, Sigman M, Thomas AJ, Schlegel PN, Howards SS, Nehra A, Damewood MD, Overstreet JW, Sadovsky R: Best practice policies for male infertility. Fertil Steril. 2002, 77: 873-882.View ArticlePubMedGoogle Scholar
- Bardaweel SK, Shehadeh M, Suaifan G, Kilani MVZ: Complementary and alternative medicine utilization by a sample of infertile couples in Jordan for infertility treatment: clinics-based survey. BMC Complement Altern Med. 2013, 13: 35-39.View ArticlePubMedPubMed CentralGoogle Scholar
- World Health Organization: WHO Laboratory Manual for the Examination of Human Semen and Semen-Cervical Mucus Interaction. 1999, Cambridge: Cambridge University Press, 1-86. 4Google Scholar
- Agarwal A, Makker K, Sharma R: Clinical relevance of oxidative stress in male factor infertility: an update. Am J Reprod Immunol. 2008, 59: 2-11.View ArticlePubMedGoogle Scholar
- Agarwal A, Sharma RK, Nallella KP, Thomas AJ, Alvarez JG, Sikka SC: Reactive oxygen species as an independent marker of male factor infertility. Fertil Steril. 2006, 86: 878-885.View ArticlePubMedGoogle Scholar
- de Lamirande E, Gagnon C: Impact of reactive oxygen species on spermatozoa: a balancing act between beneficial and detrimental effects. Hum Reprod. 1995, 10: 15-21.View ArticlePubMedGoogle Scholar
- Agarwal A, Saleh RA: Role of oxidants in male infertility: rationale, significance, and treatment. Urol Clin North Am. 2002, 29: 817-827.View ArticlePubMedGoogle Scholar
- Agarwal A, Said TM: Carnitines and male infertility. Reprod Biomed Online. 2004, 8: 376-384.View ArticlePubMedGoogle Scholar
- Aitken RJ, De Iuliis GN: Origins and consequences of DNA damage in male germ cells. Reprod Biomed Online. 2007, 14: 727-733.View ArticlePubMedGoogle Scholar
- Aitken RJ, Baker MA: Oxidative stress, sperm survival and fertility control. Mol Cell Endocrinol. 2006, 250: 66-69.View ArticlePubMedGoogle Scholar
- Sikka SC: Relative impact of oxidative stress on male reproductive function. Curr Med Chem. 2001, 8: 851-862.View ArticlePubMedGoogle Scholar
- Aziz N, Saleh RA, Sharma RK, Lewis-Jones I, Esfandiari N, Thomas AJ, Agarwal A: Novel association between sperm reactive oxygen species production, sperm morphological defects, and the sperm deformity index. Fertil Steril. 2004, 81: 349-354.View ArticlePubMedGoogle Scholar
- Alvarez JG, Storey BT: Differential incorporation of fatty acids into and peroxidative loss of fatty acids from phospholipids of human spermatozoa. Mol Reprod Dev. 1995, 42: 334-346.View ArticlePubMedGoogle Scholar
- Drevet JR: The antioxidant glutathione peroxidase family and spermatozoa: a complex story. Mol Cell Endocrinol. 2006, 250: 70-79.View ArticlePubMedGoogle Scholar
- Ebesunun MO, Solademi BA, Shittu OB, Anetor JI, Onuegbu JA, Olisekodiaka JM, Agbedana EO, Onyeaghala AA: Plasma and semen ascorbic levels in spermatogenesis. West Afr J Med. 2004, 23: 290-293.PubMedGoogle Scholar
- Rossato M, Mariotti C: Normal spermatogenesis and sperm function in a subject affected by cerebellar ataxia due to congenital vitamin E deficiency. Andrologia. 2014, 46: 322-324.View ArticlePubMedGoogle Scholar
- Alvarez JG, Storey BT: Taurine, hypotaurine, epinephrine and albumin inhibit lipid peroxidation in rabbit spermatozoa and protect against loss of motility. Biol Reprod. 1983, 29: 548-555.View ArticlePubMedGoogle Scholar
- Mancini A, Festa R, Silvestrini A, Nicolotti N, Di Donna V, La Torre G, Pontecorvi A, Meucci E: Hormonal regulation of total antioxidant capacity in seminal plasma. J Androl. 2009, 30: 534-540.View ArticlePubMedGoogle Scholar
- Mancini A, De Marinis L, Littarru GP, Balercia G: An update of Coenzyme Q10 implications in male infertility: biochemical and therapeutic aspects. Biofactors. 2005, 25: 165-174.View ArticlePubMedGoogle Scholar
- Health Quality Ontario: In vitro fertilization and multiple pregnancies: an evidence-based analysis. Ont Health Technol Assess Ser. 2006, 6: 1-63.Google Scholar
- Kamischke A, Nieschlag E: Analysis of medical treatment of male infertility. Hum Reprod. 1999, 14: 1-23.View ArticlePubMedGoogle Scholar
- Showell MG, Brown J, Yazdani A, Stankiewicz MT, Hart RJ: Antioxidants for male subfertility. Cochrane Database Syst Rev. 2011, 1: CD007411-PubMedGoogle Scholar
- Martin-Du Pan RC, Sakkas D: Are antioxidants useful in the treatment of male infertility?. Hum Reprod. 1998, 13: 2984-2985.View ArticlePubMedGoogle Scholar
- Agarwal A, Prabakaran S, Allamaneni SS: What an andrologist/urologist should know about free radicals and why. Urology. 2006, 67: 2-8.View ArticlePubMedGoogle Scholar
- Jomova K, Valko M: Advances in metal-induced oxidative stress and human disease. Toxicology. 2011, 283: 65-87.View ArticlePubMedGoogle Scholar
- Wroblewski N, Schill WB, Henkel R: Metal chelators change the human sperm motility pattern. Fertil Steril. 2003, 79: 1584-1589.View ArticlePubMedGoogle Scholar
- Donnelly ET, McClure N, Lewis S: Antioxidant supplementation in vitro does not improve human sperm motility. Fertil Steril. 1999, 72: 484-495.View ArticlePubMedGoogle Scholar
- Twigg J, Irvine DS, Houston P, Fulton N, Michael L, Aitken RJ: Iatrogenic DNA damage induced in human spermatozoa during sperm preparation: protective significance of seminal plasma. Mol Hum Reprod. 1998, 4: 439-445.View ArticlePubMedGoogle Scholar
- Crimmel AS, Conner CS, Monga M: Withered Yang: a review of traditional Chinese medical treatment of male infertility and erectile dysfunction. J Androl. 2001, 22: 173-182.PubMedGoogle Scholar
- Gerhard I, Wallis E: Individualized homeopathic therapy for male infertility. Homeopathy. 2002, 91: 133-144.View ArticlePubMedGoogle Scholar
- Ahmed RS, Seth V, Banerjee BD: Influence of dietary ginger (Zingiber officinales Rosc) on antioxidant defense system in rat: comparison with ascorbic acid. Indian J Exp Biol. 2000, 38: 604-606.PubMedGoogle Scholar
- Gerber GS: Saw palmetto for the treatment of men with lower urinary tract symptoms. J Urol. 2000, 163: 1408-1412.View ArticlePubMedGoogle Scholar
- Gerber GS, Kuznetsov D, Johnson BC, Burstein JD: Randomized, double-blind, placebo-controlled trial of saw palmetto in men with lower urinary tract symptoms. Urology. 2001, 58: 960-964.View ArticlePubMedGoogle Scholar
- Fu Y, Ji LL: Chronic ginseng consumption attenuates age-associated oxidative stress in rats. J Nutr. 2003, 133: 3603-3609.PubMedGoogle Scholar
- Hong B, Ji YH, Hong JH, Nam KY, Ahn TY: A double-blind crossover study evaluating the efficacy of Korean red ginseng in patients with erectile dysfunction: a preliminary report. J Urol. 2002, 168: 2070-2073.View ArticlePubMedGoogle Scholar
- Murphy LL, Lee TJ: Ginseng, sex behavior, and nitric oxide. Ann N Y Acad Sci. 2002, 962: 372-377.View ArticlePubMedGoogle Scholar
- Chen JC, Chen LD, Tsauer W, Tsai CC, Chen BC, Chen YJ: Effects of Ginsenoside Rb2 and Rc on inferior human sperm motility in vitro. Am J Chin Med. 2001, 29: 155-160.View ArticlePubMedGoogle Scholar
- Tamler R, Mechanick JI: Dietary supplements and nutraceuticals in the management of andrologic disorders. Endocrinol Metab Clin North Am. 2007, 36: 533-552.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1472-6882/14/244/prepub
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