A potential anti-tumor herbal medicine, Corilagin, inhibits ovarian cancer cell growth through blocking the TGF-β signaling pathways
- Luoqi Jia†1,
- Hongyan Jin†1,
- Jiayi Zhou1,
- Lianghua Chen2,
- Yiling Lu3,
- Yanlin Ming2 and
- Yinhua Yu1Email author
© Jia et al; licensee BioMed Central Ltd. 2013
Received: 12 June 2012
Accepted: 11 February 2013
Published: 15 February 2013
Phyllanthus niruri L. is a well-known hepatoprotective and antiviral medicinal herb. Recently, we identified Corilagin as a major active component with anti-tumor activity in this herbal medicine. Corilagin is a member of the tannin family that has been discovered in many medicinal plants and has been used as an anti-inflammatory agent. However, there have been few reports of the anti-tumor effects of Corilagin, and its anti-tumor mechanism has not been investigated clearly. The aim of the present study is to investigate the anticancer properties of Corilagin in ovarian cancer cells.
The ovarian cancer cell lines SKOv3ip, Hey and HO-8910PM were treated with Corilagin and analyzed by Sulforhodamine B (SRB) cell proliferation assay, flow cytometry, and reverse phase protein array (RPPA). Corilagin was delivered intraperitoneally to mice bearing SKOv3ip xenografts.
Corilagin inhibited the growth of the ovarian cancer cell lines SKOv3ip and Hey, with IC50 values of less than 30 μM, while displaying low toxicity against normal ovarian surface epithelium cells, with IC50 values of approximately 160 μM. Corilagin induced cell cycle arrest at the G2/M stage and enhanced apoptosis in ovarian cancer cells. Immunoblotting assays demonstrated that Cyclin B1, Myt1, Phospho-cdc2 and Phospho-Weel were down-regulated after Corilagin treatment. Xenograft tumor growth was significantly lower in the Corilagin-treated group compared with the untreated control group (P <0.05). More interestingly, Corilagin inhibited TGF-β secretion into the culture supernatant of all tested ovarian cancer cell lines and blocked the TGF-β-induced stabilization of Snail. In contrast, a reduction of TGF-β secretion was not observed in cancer cells treated with the cytotoxic drug Paclitaxel, suggesting that Corilagin specifically targets TGF-β secretion. Corilagin blocked the activation of both the canonical Smad and non-canonical ERK/AKT pathways.
Corilagin extracted from Phyllanthus niruri L. acts as a natural, effective therapeutic agent against the growth of ovarian cancer cells via targeted action against the TGF-β/AKT/ERK/Smad signaling pathways.
KeywordsCorilagin Herbal medicine TGF-β Ovarian cancer Epithelial-mesenchymal transition Cell cycle G2/M arrest Apoptosis
Ovarian cancer is the most common form of gynecologic neoplasm and the fifth most common cause of cancer mortality in women. Although there have been improvements in surgical techniques and treatment options, the five-year survival for stages IIB to IV ovarian cancer is less than 40% . The current chemotherapeutic in common clinical use is platinum combined with Paclitaxel, which has enhanced drug toxicity. Therefore, researchers are searching for new anti-ovarian cancer drugs that are eutherapeutic and inflict fewer side effects. Work in herbal medicine is especially highlighted.
Since 2005, we have screened hundreds of herbs, among which Phyllanthus niruri L. has the greatest anti-cancer potential. Phyllanthus niruri L. (P. niruri L.) belongs to the Euphorbiaceae family and originated in India. It usually occurs as a winter weed throughout the tropic and subtropic parts of the globe, including China, South Asia, and America. Our garden has introduced and domesticated this plant since the 1960s. In this study, whole P. niruri plants were collected from Gulangyu Islet, Fujian province, China, in October 2006 and identified by Professor Yong-Tian Zhang, Fujian Institute of Subtropical Botany, China. A voucher specimen (20061026) was deposited at Xiamen Overseas Subtropical Plant Introduction Garden, China. P. niruri L. is a popular folk medicine for treating nephritic, urocystic, gastrointestinal, and hepatic infections. It has traditionally been used in antiviral, antioxidant, anti-inflammatory, and antidiabetic treatments as well as for radiation protection. Our recent work identified that Corilagin is a major active compound from P. niruri L. extracts; it is effective in retarding the growth of hepatocarcinoma cells [unpublished data, Ming et al.].
There has been little research on the effect of Corilagin on cancer; much of the current research on Corilagin focuses on its use as an antiviral, hypolipemic, hypotensive and anticoagulation agent [2, 3]. A study from Hau DKet al. showed that Corilagin is considerably effective at retarding the in vivo growth of xenografted Hep3B hepatocellular carcinoma cells ; however, there are few reports on the pharmacology and molecular mechanism of Corilagin. When screening plant extracts for TNF-α inhibitors, Okabe et al. and Fujiki et al. found that Corilagin could significantly inhibit the secretion of TNF-α.
In this study, we investigated the effect of Corilagin on ovarian cancer cells both in vitro and in vivo. We further explored the intracellular mechanisms involving Corilagin in multiple signaling pathways and in inflammatory factor secretion.
Cell culture and reagents
The human ovarian cancer cell lines SKOv3ip and Hey were obtained from the M. D. Anderson Cancer Center (Houston, TX, U.S.A.). HO8910PM, a highly metastatic ovarian cancer cell line , was obtained from the Chinese Academy of Sciences (Shanghai, China). These cell lines were cultured in DMEM or RPMI 1640 medium supplemented with 10% fetal bovine serum. To study the correlation of Snail and TGF-β, we transfected the Snail expression vector into HO8910PM cells, thereby producing a stable Snail-expressing cell line, which was cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum and 400 μg/ml of G418. Nonmalignant ovarian surface epithelial (OSE) cells were obtained by lightly scraping the ovarian epithelial surface, followed by culture in medium 199:105 supplemented with 15% fetal bovine serum and 10 ng/ml EGF (Sigma, St. Louis, MO), as previously described . All samples were obtained with the patient’s informed consent using protocols and procedures approved by the Institutional Review Board at the Obstetrics and Gynecology Hospital of Fudan University. The antibodies against pAKT, AKT, pERK, ERK and Snail and the Cell Cycle Regulation Antibody Sampler Kit II were purchased from Cell Signaling Technology (Danvers, MA), and an anti-GAPDH antibody was purchased from Kang Chen Bio Co. (Shanghai, China). TGF-β1 was purchased from Sigma.
Extraction and purification of corilagin
Cell proliferation assay
Sulforhodamine B (SRB) was used to detect the effect of drugs on the proliferation of ovarian cancer cell lines and OSE cells. Cancer cells (1.5-2.0×103 cells per well in 100 μl medium) and OSE cells (3.0×103 cells per well in 100 μl medium) were seeded in 96-well plates and incubated with Corilagin (0, 8, 16, 24, 32, or 40 μM for the cancer cells and 0, 60, 120, 240, or 480 μM for the OSE cells) starting the following day and continuing for 3 days. After 72 hours, 50 μl of 30% trichloroacetic acid was added and incubated for 60 min at 4°C. After washing and drying the plate, 100 μl of 0.4% SRB was added for 30 min. The plates were rinsed with 0.1% acetic acid and air-dried, after which 100 μl of Tris base (10 mM/L) was added, and the plates were shaken for 5 min. The SRB value was measured at a wavelength of 490 nm. The experiment was performed in quintuplicate and repeated three times.
Cell cycle analysis
SKOv3ip and Hey cells were seeded in 60-mm plates (1–2×105/plate) and incubated with Corilagin (20–40 μM) or DMSO as a control the next day. Control and treated cells were trypsinized at 24 or 48 hours after treatment, collected in PBS and fixed on ice, followed by washing with 70% cold ethanol. After treatment with 10 μg/ml RNase, cells were stained with 50 μg/ml propidium iodide (PI, Sigma) for 15 min at room temperature in preparation for cell cycle analysis. Stained cells were analyzed by flow cytometry (FACS Calibur) (BD, Franklin Lakes, NJ). The cell cycle information was analyzed using ModFit3.0 software.
Hey cells were seeded in a 60-mm dish (1–2×105/dish) and incubated with Corilagin (20–40 μM) or DMSO as a control. Control and treated cells were trypsinized at 24 and 48 hours, collected in PBS and stained with Annexin V and PI according to the manufacturer’s instructions for the Vybrant ® Apoptosis Assay Kit (Invitrogen). The stained cells were analyzed by flow cytometry.
Reverse phase protein array (RPPA) analysis
Untreated and Corilagin-treated HO8910PM cells were used for RPPA analysis at The University of Texas, M.D. Anderson Cancer Center RPPA Core Facility. We followed the methods described at the following web address: http://www.mdanderson.org/education-and-research/resources-for-professionals/scientific-resources/core-facilities-and-services/functional-proteomics-rppa-core/index.html.
Western blot analysis
SKOv3ip cells and Hey cells were seeded in 60-mm plates (1–2×105/plate) and incubated with Corilagin (20–40 μM) or DMSO, as a control, for 24, 48 or 72 hours. Cell lysates were harvested with lysis buffer (1% Triton X-100, 50 mM Hepes, pH 7.4, 150 mM NaCl, 1.5 mM MgCl2, 1 mM EGTA, 100 mM NaF, 10 mM NaPPi, and 10% glycerol, to which 1 mM PMSF, 1 mM Na3VO4, and 1X protease inhibitor were added before use). HO8910PM-snail cells were seeded in a 60-mm plate and treated with TGF-β1 alone or in combination with Corilagin; DMSO was used as the control.
Proteins from total cell lysates were separated using a 10-15% SDS-PAGE gel and transferred to PVDF membranes (Millipore, Billerica, MA). The membranes were blocked, washed and incubated with specific primary antibodies. The primary antibody incubation was followed by incubation with HRP-conjugated secondary antibodies. The bands were detected with an enhanced chemiluminescence assay (PerkinElmer, Waltham, MA).
Various ovarian cancer cell lines were seeded in 60-mm plates (1-3×105/plate) and incubated with Corilagin (20, 40, or 80 μM) or DMSO. Culture supernatants were harvested after 1, 2, and 3 days to measure the concentration of TGF-β1. Hey cells (2.0×103 cells per well in 100 μl of medium) were seeded in 96-well plates and incubated with Corilagin (40 μM), Paclitaxel (40 nM), or DMSO the next day. Culture supernatants were harvested at 48 h to measure the concentration of TGF-β1. SRB was used to detect the effects of Corilagin and Paclitaxel on the proliferation of ovarian cancer cells. The concentration of TGF-β1 was measured by ELISA according to the manufacturer’s instructions (Shanghai ExCell Biology, Inc., Shanghai, China).
Growth of xenografts in nu/nu mice
All animal experiments were carried out in accordance with an animal protocol approved by the Institutional Animal Care and Use Committee of the Shanghai Tumor Institute. The effect of Corilagin on the in vivo growth of ovarian cancer xenograft tumors was evaluated using xenografts of the human ovarian cancer cell line SKOv3ip in Balb/c nu/nu mice. The SKOv3ip cells (1.5 × 106) were injected subcutaneously. Tumors were measured twice a week, and tumor volumes were calculated using the formula TV = (L × W2)/2, where L represents the longer diameter and W represents the shorter diameter. When palpable tumors had grown to a diameter of 0.3-0.5 cm, the mice were divided into four groups of six to eight, and each group received an intraperitoneal injection of either DMSO (as a control) or 5, 10, or 15 mg/kg of Corilagin. The doses of Corilagin used were in reference to the animal experiments of Hau DK’s group . The mice were treated three times per week for four weeks and were then sacrificed.
All data were subjected to statistical analysis and were reported as the mean ± standard deviation. The criterion for statistical significance was taken as P<0.05 using a two-tailed t-test and the count data were tested using chi-square criterion comparing the parameters frequency of parameters. The analyses were performed using SPSS 15.0 software.
Corilagin inhibits the growth of ovarian cancer cell lines in vitro and in vivo
Corilagin induces G2 cell cycle arrest and apoptosis
Corilagin induces G2/M cell cycle arrest in Hey and SKOv3ip cells
Corilagin inhibits the secretion of TGF-β1
Corilagin blocks multiple signaling pathways
Herbal medicines are currently attracting attention as potential cancer therapeutics and preventive agents. Phyllanthus niruri L. is a well-known medicinal plant that has been used as a hepatoprotective, antiviral, antibacterial, analgesic, antispasmodic and antidiabetic medicine; however, there are few reports describing its anti-tumor activity. Our group isolated components of Phyllanthus niruri L. by chromatographic fractionation and mass spectrometry. Of the two major isolated components (ethyl brevifolin carboxylate and Corilagin), Corilagin demonstrated better anti-tumor potential and lower toxicity in normal cells [unpublished data, Ming et al.]. Corilagin is a gallotannin that has been identified in several plants, including Phyllanthus niruri L. . Corilagin has been shown to exhibit versatile medicinal activity including anti-inflammatory effects as well as hepato-protective activity [2, 10, 11]. Recently, an anti-tumor effect on hepatocellular carcinoma was reported ; however, the anti-tumor mechanism is still unclear.
In this study, we confirmed the antitumor effect of Corilagin on ovarian cancer cells and further investigated the mechanism of this effect. Corilagin induced cell cycle arrest at the G2/M stage and enhanced apoptosis in ovarian cancer cells. Cyclin B1, Myt1, Phospho-cdc2 and Phospho-Weel were down-regulated after Corilagin treatment. Importantly, we found that Corilagin inhibited TGF-β secretion into the culture supernatant of all tested ovarian cancer cell lines and blocked the stabilization of Snail induced by TGF-β. The reduction of TGF-β secretion was specific to Corilagin treatment; Corilagin also targeted TGF-β-related signaling molecules, such as pAKT, pERK and pSmads. Other natural products, such as genistein and curcumin, can also alter the TGF-β pathway. Both of these agents can abrogate the enhancement of u-PA levels induced by TGF-β1 and also inhibit the TGF-β1-induced synthesis of fibronectin , inferring that some natural products have the potential to be effective in the treatment of cancer.
G2/M checkpoint-based anti-cancer strategies have focused on targeting and inactivating the G2/M checkpoint, thus forcing the cancer cells into mitosis with increased DNA damage and finally into mitotic catastrophe and cell death. The Cyclin B/cdc2 complex performs an important function in controlling the G2/M phase by rapidly phosphorylating the target protein to induce progression into the M phase [13, 14]. The phosphorylation and dephosphorylation of specific amino acids in cdc2 are responsible for the control of G2/M cell cycle progression by the Cyclin B1/cdc2 complex [13, 14]. More specifically, in the G2 phase, cdc2 is phosphorylated at Thr14 and Tyr15 by the protein kinases Myt1 and Wee1, thereby converting it into an inactive precursor. Consistent with these reports, in the present study, we observed that Corilagin decreases the protein level of Cyclin B1, p-cdc2 (Tyr15) in both Hey and SKOv3ip cells, which might be the molecular mechanism responsible for Corilagin’s efficacy in inducing G2/M arrest. We also observed down-regulation of p-Wee1 (Ser642) and Myt1 in Hey and SKOv3ip cells, indicating that the efficacy of Corilagin in inducing G2/M arrest in ovarian cancer cells is possibly due to the down-regulation of cdc2 and Cyclin B1 through Wee1 and Myt1 regulation.
Akt is suggested to function as a G2/M initiator. The activity of PI3K/Akt is required at multiple points during the cell cycle. Downstream functions of the PI3K/Akt pathway during G2/M transitions may include inhibition of the Chk1 G2 checkpoint protein or activation of cdc25C, which promotes cdc2 activation and entry into mitosis in primary oocytes from the starfish Asterina pectinifera. Akt was reported to inhibit Myt1 through Akt-dependent phosphorylation and down-regulation at the G2/M transition . In the present study, we observed that Corilagin inhibited both pAKT (Ser473) and Myt1 expression in Hey and SKOv3ip cells after stimulation with EGF, suggesting that the inhibition of Akt/Myt1 also contributes to the G2/M arrest resulting from Corilagin treatment. Further studies will be required to support these assumptions and to determine the role of upstream events, such as Chk1 and Chk2, in ovarian cancer cell responses to Corilagin.
Corilagin has been reported as a TNF-α-releasing inhibitor in inflammatory scenarios [5, 6]. In this study, we observed that the secretion of TGF-β was inhibited by Corilagin in a dose-dependent manner in all ovarian cancer cells evaluated, indicating that Corilagin also disturbed the expression and efficacy of TGF-β. Our results further demonstrated that Corilagin not only targets the classical Smad pathway via pSmad2 but also down-regulates MAPK signaling. The thing that most intrigued us is that Corilagin treatment induced a dramatic decline in the expression of the Snail protein, especially at higher doses, which indicates that Corilagin not only exerts its effects on cell cycle control but also contributes to epithelial-mesenchymal transition (EMT) in ovarian cancer.
As with all cancer cells, ovarian cancer cells undergo an EMT to disseminate within the intraperitoneal cavity or metastasize to distant sites . TGF-β signaling plays a critical role in ovarian cancer EMT and metastasis. Ovarian cancer is thought to arise from normal ovarian surface epithelium (OSE). TGF-β has been shown to inhibit human OSE proliferation and induce apoptosis, which may prevent the over-proliferation of cells during a normal ovulatory cycle . Although TGF-β can act as a tumor suppressor by inhibiting cell proliferation in the early stages of tumor development, it can also promote metastasis in various cancer models [19, 20]. It appears that at later stages, cancer cells protect themselves and tend to acquire increasing resistance to TGF-β growth inhibitory signals, which is an important reason for the shift of TGF-β from tumor suppressor to tumor promoter . Much remains to be elucidated about how TGF-β contributes to ovarian cancer progression, particularly in the regulation of EMT. A high concentration of TGF-β has been detected in ascites, blood and other bodily fluids of ovarian cancer patients . When ovarian cancer cells were cultured, various TGF-βs, including TGF-β1, TGF-β2 and TGF-β3, induced pro-matrix metalloproteinase (MMP) secretion, the loss of cell-cell junctions, down-regulation of E-cadherin, up-regulation of N-cadherin, and the acquisition of a fibroblastoid phenotype, all of which are consistent with EMT [23–25]. In addition, our recent studies identified that TGF-β is the most important inflammatory factor in ovarian cancer. TGF-β stabilizes the protein level of Snail, an inducer of EMT, and further enhances Snail expression when combined with other inflammatory factors (Jin et al. unpublished data). However, how Corilagin has this effect on TGF-β and thus undermines the stability of Snail still needs to be elucidated.
TGF-β binds to type I (ThRI) and type II (ThRII) receptors. Upon ligand binding to ThRII, ThRI is activated and phosphorylates the receptor-regulated Smads. The phosphorylated receptor-regulated Smads then bind to the co-Smad, Smad4, and translocate to the nucleus to modulate gene expression. TGF-β also initiates Smad-independent pathways, including those mediated by the mitogen-activated protein kinase family members (TAK1, extracellular signal-regulated kinase, p38, and c-Jun-NH2-kinase) and phosphatidylinositol 3-kinase . In this study, we found that Corilagin not only inhibits the secretion of TGF-β but also blocks the TGF-β-related signaling proteins pSmads, pAKT, and pERK (Figure 8). Our research provides evidence that TGF-β/Smad/AKT/ERK signaling is the target of Corilagin and that this herbal medicine could be an effective ovarian cancer therapeutic agent.
Corilagin is a major active component with anti-tumor activity from P. niruri L. Our results indicated that Corilagin distinctly inhibited the growth of ovarian cancer cells in vitro and in vivo, while displaying low toxicity against normal cells. More interestingly, Corilagin inhibited TGF-β secretion and blocked the stabilization of Snail that is induced by TGF-β; Corilagin blocked the activation of both canonical Smad and non-canonical ERK/AKT pathways. Corilagin, therefore, acts as a natural, effective therapeutic agent against the growth of ovarian cancer cells via targeted action on the TGF-β/AKT/ERK/Smad signaling pathways.
- P. niruri L Phyllanthus niruri:
Ovarian surface epithelial
Reverse phase protein array
Medium Pressure Liquid Chromatography
High Performance Liquid Chromatography.
This work was supported by funds from the National Cancer Institute (Grant number: CA 80957 to YY), the National Natural Science Foundation of China (Grant number: 81072129 to HJ, Grant number: 81274149 to YM), the Shanghai Leading Academic Discipline Project (Grant number: B117 to HJ), the Shanghai Science and Technology Commission (Grant number: 10ZR1405000 to HJ) and the Shanghai International Collaboration Program (Grant number: 10410700500 to HJ). This work was also supported by grants from the Natural Science Foundation of Fujian Province (Grant number: 2010D012 to YM) and the Xiamen Municipal Science and Technology Innovation Fund Project (Grant number: 3502Z20101016 to YM).
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