Antioxidant and phytochemical properties of Carpobrotus edulis (L.) bolus leaf used for the management of common infections in HIV/AIDS patients in Eastern Cape Province
© Omoruyi et al.; licensee BioMed Central Ltd. 2012
Received: 20 July 2012
Accepted: 4 November 2012
Published: 9 November 2012
Carpobrotus edulis (Mesembryanthemaceae), also known as igcukuma in Xhosa language is a medicinal plant used by the traditional healers to treat common infections in HIV/AIDS patients. Based on this information, we researched on the plant phytoconstituents, as well as its inhibitory effect using aqueous and three different organic solvent extracts in order to justify its therapeutic usage.
Antioxidant activity of the extracts were investigated spectrophotometrically against 1,1- diphenyl-2-picrylhydrazyl (DPPH), 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) diammonium salt, hydrogen peroxide (H2O2), nitric oxide (NO), and ferric reducing power, Total phenols, flavonoids, flavonols, proanthocyanidins, tannins, alkaloids and saponins were also determined using the standard methods.
Quantitative phytochemical analysis of the four solvent extracts revealed a high percentage of phenolics (55.7 ± 0.404%) in the acetone extract, with appreciable amount of proanthocyanidins (86.9 ± 0.005%) and alkaloids (4.5 ± 0.057%) in the aqueous extract, while tannin (48.9 ± 0.28%) and saponin (4.5 ± 0.262%) were major constituents of the ethanol extract. Flavonoids (0.12 ± 0.05%) and flavonols (0.12 ± 0.05%) were found at higher level in the hexane extract in comparison with the other extracts. The leaf extracts demonstrated strong hydrogen peroxide scavenging activity, with the exception of water and ethanol extracts. IC50 values of the aqueous and ethanolic extract against DPPH, ABTS, and NO were 0.018 and 0.016; 0.020 and 0.022; 0.05 and 0.023 mg/ml, respectively. The reducing power of the extract was found to be concentration dependent.
The inhibitory effect of the extracts on free radicals may justify the traditional use of this plant in the management of common diseases in HIV/AIDs patients in Eastern Cape Province. Overall, both aqueous and ethanol were found to be the best solvents for antioxidant activity in C. edulis leaves.
KeywordsCarpobrotus edulis Solvent extraction Antioxidant Free radicals Phytoconstituents
Living cells are known to generate free radicals reactive oxygen species (ROS) through physiological and biochemical processes in the body system [1–3]. Free radicals such as OH-, O2.-, .NO-, RO2- and LOO- are products of normal metabolic processes in the human body. It is true that the body can handle free radicals, but if these radical productions become excessive, it could cause cell wall and DNA damage, leading to chronic diseases like cancers and cardiovascular disease [1–3].
Dietary antioxidant from food intake, such as vitamin E, selenium and polyphenols like green tea has been reported to decrease the adverse effects of free radicals . They act as scavengers by donating one of their own electrons in order to replace the stolen electron from free radicals .
Several standard established antioxidant drugs such as butylhydroxytoluene (BHT) and rutin have been reported to be toxic to living cells [5–7]. Rutin drugs also known as quercetin rutinoside is a glycoside of the bioflavonoids used in many countries, including South Africa as medications for the treatment of inflammatory disorders, allergies and viruses . However, specific carcinogenic toxicity has been observed . These include swelling of the throat, tongue, lips or face, chest pain, skin rash etc. BHT drugs, are known to be the most prevalent and approved antioxidant scavengers worldwide, have equally been reported to be toxic to the lungs, even at a lower concentration [5–7].
In recent years, there has been an increasing interest in finding natural antioxidants from medicinal plants . Plants are endowed with free radical scavenging molecules, such as vitamins, terpenoids, phenolic acids, tannins, flavonoids, alkaloids, and other metabolites, which are rich in antioxidant and free radical scavenging properties . In addition, the ingestion of natural antioxidants has shown to enhance the immune defence, reduce risks of cancer, cardiovascular disease, diabetes, and other diseases associated with ageing [12, 13].
Carpobrotus edulis (L.) Bolus (Mesembryanthemaceae), also known as igcukuma in Xhosa communities, is an edible easily grown groundcover plant that is widespread in the Eastern Cape of South Africa. It flourishes on sandy soil with thick greenish succulent leaves reaching about 10.8 cm in length. This plant is used by the traditional healers in the above mentioned province to treat tuberculosis, diabetes mellitus, sores, high blood pressure, intestinal worms and constipation. It is possible that this plant may contain some bioactive secondary metabolites that work against opportunistic infections .
The antioxidant action of polyphenol compounds depends on their free radical scavenging capacity and its ability to reduce iron . The total polyphenol amounts determined from plant and their corresponding antioxidant activity may vary widely, depending on the extraction solvents applied. For example, aqueous and acetone showed the highest efficiency for extraction of phenols among the various solvents used . Olubunmi and Anthony , reported that acetone on its own has the capability of extracting both the polar and non-polar compounds from plant samples. Similarly, Brenes et al.  reported that the use of acetone extraction solvent resulted in a complete extraction of phenols from olive oils, when compared with other solvent extracts. Methanol extracts however showed the highest antioxidant activities in seabuckthorn seeds when compared with chloroform and ethyl acetate solvent . Recently, we compared the polyphenol content from various solvents (aqueous, ethanol, acetone and hexane) extract of C. edulis leaf. Aqueous and ethanol were found to be the best solvents for antioxidant activity .
Limited information exists on antioxidant activity of Carpobrotus edulis (L) Bolus. Hanen et al.  examined the phytochemical properties of methanolic extracts using different plant parts including the leaf of C. edulis but no findings are recorded for the antioxidant activity of the aqueous, ethanol, acetone and hexane extracts considering that successful isolation of bio-compounds from plant material is largely dependent on the type of solvent used in the extraction procedure . Therefore, the aim of this study were (1) to determine the quantitative phytochemical present in various extraction solvents of varying polarities, (2), to determine their antioxidant activities in comparison to the established standard drugs in order to justify its therapeutic usage.
Collection and preparation of the extracts
Fresh leaves of C. edulis were collected from the Alice area in the Eastern Cape, SA. The plant was authenticated by Prof. DS Grierson of the Botany Department, University of Fort Hare, where a voucher specimen (Omo 2011/1-Omo 2011/19) was kept.
The leaves were washed with tap water, oven dried at 50°C for 24 h and ground to fine power using a electric blender (Waring Products Division, Torrington, USA). Hundred grams (100.00 g) of finely ground plant material was extracted with 1 L of hexane, acetone, ethanol and distilled water respectively. The containers and contents were vigorously shaken for 48 h (Stuart Scientific Orbital SOI, Essex). Particulate matter was allowed to sediment and the supernatant was filtered using Buchner funnel and Whatman No. 1 filter paper. This process was repeated by re-filtering the supernatant with sterile cotton wool and evaporated using a rotavaporator (R-114; Büchi, New Castle, USA) and decanted into pre-weighed labelled beakers. The different extracts were reconstituted in their various extraction solvents to give the required concentrations needed in this study.
Preliminary screening of the extract phytochemicals
Initial screening tests of the four extracts were performed to ascertain the presence or absence of phytoconstituents such as phenolic compounds, flavonoids, flavonols, proanthocyanidins, tannins, saponins, and alkaloids using standard procedure described by Alex et al. .
Determination of total phenolic content
Total phenol content in the various extracts was determined by the modified Folin-ciocalteu method of Zovko et al. . An aliquot of 0.5 ml of each extract (1 mg/ml) was mixed with 2.5 ml Folin-Ciocalteu reagent (previously diluted with distilled water 1:10 v/v) and 2 ml (75% w/v) of sodium carbonate (Na2CO3). The tubes were vortexed for 15 s and allowed to stand for 30 min at 40°C for colour development. Absorbance was then measured at 765 nm using Hewlett Packard, UV/visible light spectrophotometer. Samples of extract were evaluated at a final concentration of 1 mg/ml. Total phenolics content were expressed as mg/g tannic acid equivalent using the following equation from the calibration curve: Y = 0.1216×, R2= 0.936512, where × is the absorbance and Y is the tannic acid equivalent in mg/g. The experiment was conducted in triplicate and the results were expressed as mean ± SD values.
Estimation of total flavonoids
The formations of a complex aluminium chloride colour were estimated by using the method described by Ordonez et al. . Half a ml of various solvent extracts (1 mg/ml) was mixed with 0.5 ml of 2% aluminium chloride (AlCl3) prepared in ethanol. The resultant mixture was incubated for 60 min at room temperature for yellow colour development which indicated the presence of flavonoid. The absorbance was measured at 420 nm using UV–VIS spectrophotometer. Extract samples were evaluated at a final concentration of 1 mg/ml. Total flavonoid content was calculated as quercetin equivalent (mg/g) using the following equation based on the calibration curve: Y = 0.255x, R2 = 0.9812, where × is the absorbance and Y is the quercetin equivalent.
Estimation of total flavonols
Total flavonol content was determined by adopting the procedure described by Karunakaran and Kumaran . The reaction mixture consisting of 2 ml of the sample, 2 ml of AlCl3 prepared in ethanol and 3 ml of (50 g/l) sodium acetate solution was allowed to incubate for 2.5 h at 20°C. Absorbance at 440 nm was measured. Total flavonol content was calculated as mg/g of quercetin equivalent from the calibration curve using the equation: Y Y = 0.0255×, R2 = 0.9812 where × is the absorbance and Y is the quercetin equivalent.
Determination of total proanthocyanidins content
Determination of proanthocyanidins content was done using the procedure reported by Ashafa et al. . A volume of 0.5 ml of each extract solution was mixed with 3 ml of 4% v/v vanillin prepared in methanol and 1.5 ml of hydrochloric acid and then vortexed. The resulting mixture was allowed to stand for 15 min at room temperature followed by the measurement of the absorbance at 500 nm. Total proanthocyanidin content was expressed as catechin (mg/g) using the following equation of the curve: Y = 0.5825×, R2 = 0.9277, where × is the absorbance and Y is the catechin equivalent.
Tannin content of the samples was determined according to the modified vanillin-HCl methanol method as described by Noha et al. . The vanillin-HCL reagent was prepared by mixing equal volume of 8% HCl and 1% vanillin in methanol. The reagent was mixed just prior to use. About 0.2 g of the ground sample was placed in a small conical flask. Then 10 ml of 1% concentrated HCL in methanol was added. The flask was capped and continuously shaken for 20 min and the content was further centrifuged at 2500 rpm for 5 min. About 1.0 ml of the supernatant was pippetted into a test tube containing 5 ml of vanillin-HCL reagent.
Absorbance at 450 nm was read on spectrophotometer after 20 min of incubation at 30°C. A standard curve was prepared expressing the result as catechin equivalent as follows: Tannin (%) = C × 10 × 100/200. Where: C = Concentration corresponding to the optical density; 10 = volume of the extract (ml); 200= Sample weight (mg).
Alkaloids content of the plant sample was determined using the method described by Onyilagha and Islam . Five gram of the powdered sample was weighed into a 250 ml beaker and 200 ml of 20% acetic acid in ethanol was added and covered to stand for 4 h. This was filtered and the extract was concentrated using a water bath to one-quarter of the original volume. Concentrated ammonium hydroxide was added drop wise to the extract until the precipitation was completed. The whole solution was allowed to settle and the collected precipitates were washed with dilute ammonium hydroxide and then filtered. The residue was dried and weighed. The alkaloid content was determined using this formula; .
Ferric-reducing power (FRAP) assay
The reducing power of the extract was evaluated according to the method of Hemalatha and Kumar . The mixture containing 2.5 ml of 0.2 M phosphate buffer (pH 6.6) and 2.5 ml of potassium ferricyanide (K3Fe(CN)6 (1% w/v) was added to 1mL of each of the extracts at different concentrations ranging from 0.025 – 0.5 mg/ml. The resulting mixtures were incubated at 50°C for 30 min, followed by the addition of 2.5 ml of trichloroacetic acid (10% w/v). The mixture was centrifuged at 3000 rpm for 10 min to collect the upper layer of the solution. A volume of 2.5 ml supernatant solution was mixed with distilled water (2.5 ml) and 0.5 ml of FeCl3 (0.1%, w/v). The absorbance was then measured at 700 nm against blank sample. Ascorbic acid and butylated hydroxyl toluene solution were used as positive controls. Increased absorbance of the reaction mixture indicated higher reducing power of the plant extract.
Scavenging activity of 2, 2-Diphenyl-1-Picrylhydrazyl (DPPH) radical
The effect of extracts on DPPH radical was estimated using the method of Liyana-Pathiranan et al. . About 0.1 ml of DPPH-methanol solution (0.135 mM) was mixed with 1.0 ml of different concentrations (0.025–0.5 mg/ml) of various extracts of C. edulis. The reaction mixture was vortexed thoroughly and left in the dark at room temperature for 30 min. The absorbance of the mixture was measured spectrophotometrically at 517 nm. Rutin and Butylated hydroxyl toluene (BHT) were used as standard drugs. The percentage of free radical scavenging was calculated according to the following equation: % scavenging = 100–(Abs sample–Abs blank)/Abs Control × 100.
2, 2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid (ABTS) scavenging activity
Abscontrol is the absorbance of ABTS radical+methanol;
Abssample is the absorbance of ABTS radical+sample extract/standard.
Nitric oxide radical scavenging activity
The scavenging radical of nitric oxide was based on the procedure reported by Ebrahimzadeh et al. . A volume of 2 ml of 10 mM sodium nitroprusside dissolved in 0.5 ml phosphate buffer saline (pH 7.4) was mixed with 0.5 ml of each plant extract or BHT or rutin at various concentrations (0.025-0.5 mg/ml). The mixture was incubated at 25°C for 150 min. An aliquot of 0.5 ml of the solution was withdrawn and mixed with 0.5 mL of Griess reagents [(1.0 ml sulfanilic acid reagent (0.33% in 20% glacial acetic acid at room temperature for 5 min with 1 ml of naphthylethylenediamine dichloride (0.1% w/v)]. The reaction mixture was incubated at room temperature for 30 min, after which absorbance was measured at 540 nm. The amount of nitric oxide radical was calculated using the equation: % inhibition of NO= A0−A1/A0 × 100, where A0 is the absorbance before reaction and A1 is the absorbance after reaction has taken place.
Hydrogen peroxide scavenging activity
The ability of the plant extract to scavenge hydrogen peroxide was determined according to the method given by Karunakaran and Kumaran . A solution of 4 mM H 2 O 2 was prepared in phosphate buffer (0.1 M pH 7.4). Plant extract (4 ml) prepared in methanol at various concentrations (0.025–0.5 mg/ml) were mixed with 0.6 ml of 4 mM H 2 O 2 solution prepared in phosphate buffer. The absorbance of hydrogen peroxide at 230 nm was determined after 10 min against a blank solution containing the plant extract without H 2 O 2 . The result obtained was compared with standard ascorbic acid. Percentage inhibition of H 2 O 2 = Abs(control) – Abs(sample)/Abs(control) × 100, where, Abs (control): Absorbance of the control and Abs (sample): Absorbance of the extracts/standard.
The experimental results were expressed as mean ± standard deviation (SD) of three replicates. Where applicable, the data were subjected to one way analysis of variance (ANOVA) to determine the significance difference in the extract used.
Results and discussion
Results of phytochemical screening of the extracts from C. edulis leaf
Quantification of polyphenolic compounds
Due to the vast differences in the nature of the phytochemical constituents found in a plant, there is no particular solvent that is known to extract all the compounds on its own from the plant . Therefore, in this study, we considered using hexane, acetone, ethanol and aqueous solvents for extraction to accommodate the range of polarities of the compounds present in C. edulis leaves. Our results showed that the choice of these various solvents played a crucial role in the quantitative analysis of different polyphenols extracted from the plant samples. The yield of different solvent extracts of C. edulis leaf is presented in Table 1.
Quantitative analysis of the phytochemical evaluated from the leaf of C. edulis
Amount of phytochemical compounds in (mg/g)
517.71 ± 0.40 a٭
330.87 ± 0.04 b
557.11 ± 0.23 a
64.14 ± 0.15 b
0.29 ± 0.01 b
0.28 ± 0.01 b
0.65 ± 0.04 b
1.19 ± 0.041 b
0.05 ± 0.001 a
0.05 ± 0.001 a
0.23 ± 0.05 a
0.19 ± 0.03 a
896.7 ± 0.05 b
115.28± 0.007 a
753.87 ± 0.02 b
134.91 ± 0.01 a
461 ± 0.07 a
489 ± 0.28 b
384 ± 0.14 a
64 ± 0.14 b
34 ± 0.21 a
45 ± 0.26 a
11 ± 0.071 b
2 ± 0.035 b
45 ± 0.06 b
38 ± 0.02 b
31 ± 0.021 b
3 ± 0.014 b
Quantification of compounds obtained from the crude extract varied greatly among the four solvents, which is an indication that solvents have different extracting capacity for polyphenols. As shown in Table 1, the concentration of phenol in the four solvent extracts is in the following decreasing order: acetone < aqueous < ethanol < hexane. The observed high phenol content in acetone (557.11 ± 0.228 mg/g) and aqueous (517.71 ± 0.015 mg/g) fractions is in agreement with what has been reported by other researchers . However, there was no significant difference between acetone and water extracts at 95% confidence interval.
The value of flavonoid content in all the four solvent extracts was significantly different from each other at 95% confidence interval, Table 1. The solvent capacity follows the decreasing order of the extract hexane < acetone < aqueous < ethanol. Our results were contrary to the report of Syeda et al.  who reported that solvent extraction of total flavonoids followed a decreasing order of ethanol < methanol < acetone < ethyl acetate < dichloromethane < hexane.
In this study, the highest value of flavonols (0.23 ± 0.050 mg/g) was obtained from acetone extract, followed by hexane extract (0.19 ± 0.030 mg/g). The variation in the extraction capacity of the solvents could be due to the polarity of the solvent used, even though the differences were not significant.
An appreciable amount of proanthocyanidin was observed respectively in both aqueous (896.7 ± 0.005 mg/g) and acetone (753.89 ± 0.017 mg/g) extracts, while a moderate concentration was formed in hexane (134.91 ± 0.014 mg/g) and ethanol (115.28 ± 0.007 mg/g) extract, but the differences was not significant. Our results suggested that acetone and water are good solvent for extraction of bioactive compounds from plants as they gave the highest yield compared with other solvent used in this investigation.
Among the four solvent extracts for total tannins content, ethanol offered the best result (48.9 ± 0.283%), followed by aqueous extract (46.1 ± 0.071%), acetone extract (38.4 ± 0.141) and hexane (6.4 ± 0.141). Similar trends were found by Hanen et al.  for the total tannin contents using methanol extract, though the results obtained in this study was higher.
The percentage concentration of saponins in the ethanol extract (4.5 ± 0.262%) was not significantly different from the aqueous extract (3.4 ± 0.21%). Alkaloid content of the various extracts followed the trend: water < ethanol < acetone < hexane extracts [Table 1]. The results showed that aqueous extract exhibited the highest concentration of alkaloids compared to other solvents.
Nevertheless, our present results on C. edulis phytochemicals showed parallel trends in the behaviour of same family species revealed by Hanen et al. .
Generally, polyphenol are known to be major plant compounds and they have been reported to have multiple biological effects, including antioxidant activity. Their antioxidant activity is mainly due to their redox properties, hydrogen donors and singlet oxygen quenchers, which can play an important role in adsorbing and neutralizing free radicals [33, 34]. The importance of the antioxidant constituents of plant materials in the maintenance of health and protection from heart disease or cancer is also raising interest among scientists, food manufacturers, and consumers .
Scavenging assay of the four solvent extracts against DPPH
At the lowest concentration of 0.025 mg/ml, aqueous extract was comparable with that of the standard drugs BHT (90 ± 0.014) with IC50 value of 0.015 mg/ml and rutin (91.4 ± 0.006) with IC50 value of 0.018 mg/ml. Thus these comparisons indicate that aqueous and ethanol extracts possess high DPPH scavenging activity at the lowest concentration (0.025 mg/ml). This is an indication that C. edulis can serve as a potential natural antioxidant over standard drugs.
Scavenging assay of the four solvent extracts against ABTS radicals
C. edulis extracts against nitric oxide activity
Acetone and hexane extracts had activity at 0.062 and 0.025 mg/ml respectively. Lower absorbance (IC50) of the reaction mixture indicates higher free radical scavenging activity . Therefore, it can be stated that ethanol extract possess strong antioxidant activity. The nitric oxide scavenging activities of the four extracts decreases in this order: BHT < rutin < ethanol < aqueous < hexane < acetone extracts.
Inhibitory activity of the four extracts against hydrogen peroxide
Hydrogen peroxide (H2O2) is a well-known antimicrobial agent with cleansing property when it was first introduced into clinical practice . In recent times it has lost that favour as a result of its toxicity effect in the human cells . As a liquid, H2O2 is usually used as alternative for conventional western medicine to treat patients. Occasionally, it can also be mixed with water to cure skin infection or dirty wounds .
In summary, C. edulis extracts appear to possess compounds with antioxidant properties, thus justifying its traditional usage for the management of common infections in HIV/AIDS patients. Overall, both aqueous and ethanol were found to be the best solvents for antioxidant activity. We will conduct further research to isolate and identify the active compounds, and to determine their exact mechanism of action.
- C. edulis :
Reactive Oxygen Species
Lipid Peroxyl Radical
Analysis Of Variance
Tannic acid Equivalent
Least Significant Difference.
This work was supported by the National Research Foundation of South Africa and the Govan Mbeki Research and Development centre of the University of Fort Hare.
- Mandal M, Misra TK, Ghosal M: Free-radical scavenging activity and phytochemical analysis in the leaf and stem of Drymaria diandra Blume. Int J Integr Biol. 2009, 7: 80-84.
- Gulcin I: Antioxidant activity of food constituents: an overview. Arch Toxicol. 2012, 86: 345-391. 10.1007/s00204-011-0774-2.View ArticlePubMed
- Cetinkaya Y, Gocer H, Menzek A, Gulcin I: Synthesis and antioxidant properties of (3,4-dihydroxyphenyl)(2,3,4-trihydroxyphenyl) methanone and its derivatives. Arch Pharm (Weinheim). 2012, 345: 323-334. 10.1002/ardp.201100272.View Article
- Traber MG, Shils ME, Shike M, Ross AC, Caballero B, Cousins R: Modern nutrition in health and disease. 2006, Philadelphia: Lippincott Williams and Wilkiins, 396-411. 10
- Madhavi DL, Deshpande SS, Salunkhe DK: Toxicological aspects of food antioxidants. 1995, New York: Dekker, p 267-
- Gocer H, Gulcin I: Caffeic acid phenethyl ester (CAPE): correlation of structure and antioxidant properties. Int J Food Sci Nutr. 2011, 62: 821-825. 10.3109/09637486.2011.585963.View ArticlePubMed
- Bursal E, Gulcin I: Polyphenolcontents and in vitro antioxidant activities of lyophilised aqueous extract of kiwifruit (Actinidia deliciosa). Food Res Int. 2011, 44: 1482-1489. 10.1016/j.foodres.2011.03.031.View Article
- PDR Health - Physicians Desktop Reference: Herbals & Supplements - Rutin Drugs.com: Rutin (Bioflavonoids).http://www.livestrong.com,
- Ewa F, Ewa I, Wojciech P: Effect of pregnancy and tobacco smoke on the antioxidant activity of rutin in an animal model. Pharmacol report. 2009, 61: 935-940.View Article
- Mbaebie BO, Edeoga HO, Afolayan AJ: Phytochemical analysis and antioxidants activities of aqueous stem bark extract of Schotia latifolia Jacq. A Pac J Trop Biomed. 2012, 2: 118-124. 10.1016/S2221-1691(11)60204-9.View Article
- Ebrahimzadeh MA, Nabavi SM, Nabavi SF, Bahramian F, Bekhradnia AR: Antioxidant and free radical scavenging activity of H. officinalis, L. Var. angustifolius, V. odorata, B. hyrcana and C. speciosum. Pak J Pharm Sci. 2010, 23: 29-34.PubMed
- Yang CS, Landau JM, Huang MT, Newmark HL: Inhibition of carcinogenesis by dietary polyphenolic compounds. Annual Rev Nutr. 2001, 21: 381-406. 10.1146/annurev.nutr.21.1.381.View Article
- Sun J, Chu YF, Wu XZ, Liu RH: Antioxidant and antiproliferative activities of common fruits. J Agric Food Chem. 2002, 50: 7449-7454. 10.1021/jf0207530.View ArticlePubMed
- Smith-Palmer A, Stewart J, Fyfe L: Antimicrobial properties of plant essential oils and essences against five important food-borne pathogens. Lett Appl Microbiol. 1998, 26: 118-122. 10.1046/j.1472-765X.1998.00303.x.View ArticlePubMed
- Nihal TY, Sedat V, Ferda S, Gokce P: Effect of extraction conditions on measured total polyhenol contents and antioxidant and antibacterial activities of black tea. Molecules. 2007, 12: 484-496. 10.3390/12030484.View Article
- Olubunmi AW, Afolayan JA: Phytochemical constituents and antioxidant activities of the whole leaf extract of Aloe ferox Mill. Pharmacogn Mag. 2011, 7: 325-333. 10.4103/0973-1296.90414.View Article
- Brenes M, Garcia A, Garcia P, Garrido A: A rapid and complete extraction of phenols from olive oil and determination by means of a coulometric electrode array system. J Agric Food Chem. 2000, 48: 5178-5183. 10.1021/jf000686e.View ArticlePubMed
- Bushra S, Farooq A, Muhammad A: Effect of Extraction Solvent/Technique on the Antioxidant Activity of Selected Medicinal Plant Extracts. Molecules. 2009, 14: 2167-2180. 10.3390/molecules14062167.View Article
- Hanen F, Riadh K, Faten M, Sylvain G, Chedly A, Christian M: Antioxidant activity and phenolic composition of the medicinal and edible halophyte Mesembryanthemum edule L. Indust crops and products. 2011, 34: 1066-1071. 10.1016/j.indcrop.2011.03.018.View Article
- Pirwana K, Chokoe PM, Matlou P, Mokgotho R, Howard L, Leseilane JM: Does seasonal variation influence the phytochemical and antibacterial properties of Carpobrotus edulis. Afr J Biotechnol. 2008, 7: 4164-4171.
- Alex B, George AK, Johnson NB, Patrick A, Elvis OA, Ernest OA, Gabriel MA, Cecillia KA, Justice A, Dennis KAP: Gastroprotective effect and safety assessment of Zanthoxylum Aanthoxyloides (Lam) Waterm root bark extract. American J. Pharm and Toxicol. 2012, 7: 80-80.
- Zovko KM, Kremer D, Gruz J, Strnad M, Bisevac G, Kosalec I: Antioxidant and antimicrobial properties of Moltkia petraea (tratt.) Griseb. Flower, leaf and stem infusions. Food Chem Toxicol. 2010, 48: 1537-1542. 10.1016/j.fct.2010.03.021.View Article
- Ordonez AAL, Gomez JD, Vattuone MA, Isla MI: Antioxidant activities of Sechium edule (Jacq). Food Chem. 2006, 97: 452-458. 10.1016/j.foodchem.2005.05.024.View Article
- Karunakaran RJ, Kumaran A: In vitro antioxidant activities of methanol extract of five phyllanthus species from India. LWT. 2007, 40: 344-353. 10.1016/j.lwt.2005.09.011.View Article
- Ashafa AOT, Sunmonu O, Afolayan AJ: Effects of leaf and berry extracts of Phytolacca dioica L on haematological and weight parameters of Wistar rats. Afri J Pharmacy and Pharmacol. 2011, 5: 150-154.View Article
- Noha A, Mohammed IA, Mohamed A, Elfadil EB: Nutritional Evaluation of Sorghum Flour (Sorghum bicolor L. Moench) During Processing of Injera. World Academy of Sci Engin and, Technol. 2011, 75: 86-112.
- Okwu DE, Josiah C: Evaluation of the chemical composition of two Nigerian medicinal plants. Afr J Biotech. 2006, 5: 357-361. Fullh
- Onyilagha JC, Islam S: Flavonoids and other polyphenols of the cultivated species of the genus Phaseolus. Int J Agric Biol. 2009, 11: 231-234.
- Hemalatha S, Kumar RS: In vitro antioxidant activity of alcoholic leaf extract and subfractions of Alangium lamarckii Thwaites. J Chem Pharm Res. 2011, 3: 259-267.
- Liyana-Pathiana CM, Shahidi F: Antioxidant activity of commercial soft and hard wheat (Triticum aestivium L) as affected by gastric pH conditions. J Agric Food Chem. 2005, 53: 2433-2440. 10.1021/jf049320i.View Article
- Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C: Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biol and Med. 1999, 26: 1231-1237. 10.1016/S0891-5849(98)00315-3.View Article
- Syeda B, Bukhari MIB, Shahabuddin M: Antioxidative Activity of Extracts from Fenugreek Seeds (Trigonella foenum-graecum). Pak J Anal Environ Chem. 2008, 9: 78-83.
- Gulcin I, Topal F, Cakmakci R, Bilsel M, Goren AC, Erdogan U: Pomological features, nutritional quality, polyphenol content analysis, and antioxidant properties of domesticated and three wild ecotype forms of raspbarries (Rubus idaeus L.). J Food Sci. 2011, 76: C585-C593. 10.1111/j.1750-3841.2011.02142.x.View ArticlePubMed
- Gulcin I, Topal F, Ozturk Sarikaya SB, Bursal E, Birsel G, Goren AC: Polyphenol contents and antioxidant properties of Medlar (Mespilu germanica L.). Rec Nat Prod. 2011, 5: 158-175.
- Yazdanparast R, Bahramikias S, Ardestani A: Nasturtium oficinale reduces oxidative stress and enhances antioxidant capacity in hypercholesterolaemic rats. Chem Biol Interact. 2008, 172: 176-184. 10.1016/j.cbi.2008.01.006.View ArticlePubMed
- Park EJ, Jhon DY: The antioxidant, angiotensin converting enzyme inhibition activity, and phenolic compounds of bamboo shoot extracts. LWT – Food Sci Technol. 2010, 43: 655-659. 10.1016/j.lwt.2009.11.005.View Article
- Mohamed HM, Ons ET, Yosra S, Rayda G, Moncef N: Chemical composition and antioxidant and radical-scavenging activities of periploca laevigata root bark extracts. J Sci and Food and Agricultural. 2008, 89: 897-905.View Article
- Hashasa MM, Abdel-Gawad MM, El-Sayed MM, Sabry WA, Abdel-Hameed ES, Abdel-Lateef EE: Antioxidant properties of methanolic extracts of the leaves of seven Egyptian Cassia species. Acta Pharmaceutica. 2010, 60: 361-367. 10.2478/v10007-010-0030-y.
- Gulcin I: Antioxidant activity of eugenol: a structure-activity relationship study. J Med Food. 2011, 14: 975-985. 10.1089/jmf.2010.0197.View ArticlePubMed
- Shu-Dong W, Hai-Chao Z, Yi-Ming L: Antioxidant Activities of Extract and Fractions from the Hypocotyls of the Mangrove Plant Kandelia candel. Int J Mol Sci. 2010, 11: 4080-4093. 10.3390/ijms11104080.View Article
- Banerjee S, Chanda A, Ghoshal A, Debnath R, Chakraborty S, Saha R, Das A: Nitric Oxide Scavenging Activity Study of Ethanolic Extracts of from Two Different Areas of Kolkata Ixora coccinea. Asian J Exp Biol. 2011, 2: 595-599.
- Ndip R, Malange AE, Echakachi CM, Malongue A, Akoachere JFTK, Ndip LM, Luma HN: In vitro antimicrobial activity of selected honeys on clinical isolates of Helicobacter pylori. Afr Health Sci. 2007, 7: 228-231.PubMed CentralPubMed
- Mohammed FAG, Nagendra PK, Kong KW, Amin I: Flavonoid, hesperidine, total phenolic contents and antioxidant activities from Citrus species. Afr J Biotechnol. 2010, 9: 326-330.
- Fang YZ, Yang S, Wu G: Free radicals, antioxidants, and nutrition. Nutrition. 2002, 18: 872-879. 10.1016/S0899-9007(02)00916-4.View ArticlePubMed
- Kirmizigul S, Boke N, Sumbul H, Gokturk RS, Arda N: Essential fatty acid components and antioxidant activities of eight Cephalaria species from south-western Anatolia. Pure and Applied Chemistry. 2007, 79: 2297-2304. 10.1351/pac200779122297.View Article
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1472-6882/12/215/prepub
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