Fig. 6

APHD activates the Keap1/Nrf2/HO-1 signaling cascade in damage to PC12 cells induced by OGD/R. APHD increased the mRNA concentrations of Nrf2 (A) and HO-1 (B), as well as the protein concentrations of Keap1, Nrf2, and HO-1 (C–F). APHD promotes Nrf2 nuclear translocation (G), as detected by immunofluorescence assay. Nrf2 was labeled with specific antibody (green) and nuclei with DAPI (blue). Representative images of merged and enlarged views are displayed. Scale bar = 50 μm, and the magnification of merge is 400×. Treatment with ZnPP, an HO-1 inhibitor, reversed the antioxidant effect of APHD (H). Cell viability changes after Nrf2 siRNA interference (I). Data have the expressions of mean ± SD, n = 3. Distinct letters (a, b, c) represent significant differences at p < 0.05 employ the Compact Letter Display (CLD) methodology