Fig. 5

CK induced ferroptosis of HepG2 and SK-Hep-1 cells by regulating the FOXO signaling pathway. (A) Western blot was used to detect FOXO pathway-related proteins in cells after 48 h treatment with CK (20 µM, 40 µM, 60 µM) and 5-Fu (10 µM). (B) Western blot of ferroptosis-related proteins GPX4 and SLC7A11 in HepG2 and SK-Hep-1 cells treated with AS1842856 (0.3 µM) and CK (40 µM). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, compared with the vehicle group; ^#P < 0.05, ^##P < 0.01, compared with the CK + AS1842856 group and ferrostatin-1 group. Pairwise comparison between groups was performed by t-test