Fig. 3

Effect of ferrostatin-1 and CK on the ferroptosis pathway in HepG2 and SK-Hep-1 cells. (A) The FerroOrange probe was used to detect Fe²⁺ levels in HepG2 and SK-Hep-1 cells (400×). (B) Liperfluo cell lipid peroxide probes were used to examine cells treated with CK (40 µM) and ferrostatin-1 (2 µM). The lipid peroxidation rate of HepG2 and SK-Hep-1 cells was observed by fluorescence microscopy after 48 h (400×). (C, D) Effects of ferrostatin-1 (2 µM) and CK (40 µM) on MDA and GSH contents, compared with vehicle group, ^*P < 0.05, ^**P < 0.01. (E, F) The expression of GPX4 and SLC7A11 after CK and ferrostatin-1 treatment in cells. ^*P < 0.05, ^**P < 0.01, compared with the vehicle group; ^#P < 0.05, ^##P < 0.01, the ferrostatin-1 group was compared with the CK + ferrostatin-1 group. Pairwise comparison between groups was performed by t-test