Table 1 The primers used for RT-qPCR assay
Genes | Primers | Sequences (5’→3’) |
|---|---|---|
BCL-2 | Forward | CTTTGAGTTCGGAGGGGTCA |
Reverse | AAATCAAACAGGGGCCGCAT | |
BAX | Forward | GCCCTTTTGCTTCAGGGTTT |
Reverse | ACAGCTGCGATCATCCTCTG | |
CASP-3 | Forward | CTGAGGGTCAGCTCCTAGCG |
Reverse | CCAGAGTCCATTGATTTGCTTC | |
CASP-9 | Forward | GATCAGGCCAGGCAGCTAAT |
Reverse | CGGCTTTGATGGGTCATCCT | |
mTOR | Forward | AACATCACCAATGCCACCAC |
Reverse | TTGCTCTCGGCTTCACTTTC | |
LC3 | Forward | GCCTTCTTCCTGCTGGTGAA |
Reverse | TCCTGCTCGTAGATGTCCGC | |
BECN1 | Forward | TCCATGCTCTGGCCAATAAG |
Reverse | ACGGCAGCTCCTTAGATTTG | |
NEF2L2 | Forward | CCTCAAAGCACCGTCCTCAG |
Reverse | GCTCATGCTCCTTCTGTCGT | |
HO-1 | Forward | CAAGCGCTATGTTCAGCGAC |
Reverse | GCTTGAACTTGGTGGCACTG | |
β-actin | Forward | GCAGGAGTACGATGAGTCCG |
Reverse | TGTCACCTTCACCGTTCCA G |