Fig. 3

Monitoring autophagy-related proteins (Beclin-1, LC3-II/I ratio, and P62) in HT-29 cells after treatment with Wnt3a inhibitor (LGK974), TQ, and LGK974 + TQ. Data indicate the induction of autophagy response in HT-29 cells indicated by the increase of Beclin-1 and LC3-II/I ratio and reduction of P62 in the treated groups. One-way ANOVA and Tukey post hoc test (n = 3). *p < 0.05, **p < 0.01; ***p < 0.001