Fig. 1

MTT assay. The viability of human colorectal adenocarcinoma HT-29 cells was monitored using an MTT assay after 48 h (A; n = 7). Data indicated a significant reduction of HT-29 cell viability after being exposed to LGK974 (Wnt3a inhibitor) and TQ. Compared to the TQ and LGK974 groups, treatment of cells with the combination of TQ and LGK974 did not yield statistically significant results. Morphological features of cells after being treated with TQ, LGK974, and TQ + LGK974 (B). In the treated groups, loosened cell-to-cell connections led to the appearance of numerous round-shaped single cells. One-way ANOVA and Tukey post hoc test. ****p < 0.0001