Fig. 7

Triptolide rescues TNF-α-induced inhibition of osteogenesis in hPDLSCs via the p-IκBα/NF-κB pathway. (A) p-IκBα, NF-κB and p-NF-κB protein expression in hPDLSCs after osteogenic induction for 7 days under TNF-α, TNF-α (20ng/ml) and triptolide (10^− 11M), TNF-α (20ng/ml) and BAY11-7082 (10^− 5M) treatment. (B) Semi-quantitative analysis of p-IκBα protein levels in hPDLSCs. (C) Semi-quantitative analysis of p-NF-κB protein levels in hPDLSCs. (D) Relative p-NF-κB/NF-κB protein expression levels. The blots were cut prior to hybridisation with antibodies during blotting