Fig. 6

Activation of the NF-κB pathway after addition of TNF-α at different time points. (A) Western blot assay for p-NF-κB protein expression in hPDLSCs after osteogenic induction for 7 days under TNF-α (20ng/ml) treatment. (B) Semi-quantitative analysis of p-NF-κB protein levels in hPDLSCs. The blots were cut prior to hybridisation with antibodies during blotting