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Fig. 4 | BMC Complementary Medicine and Therapies

Fig. 4

From: Triptolide mitigates the inhibition of osteogenesis induced by TNF-α in human periodontal ligament stem cells via the p-IκBα/NF-κB signaling pathway: an in-vitro study

Fig. 4

Triptolide rescues TNF-α-induced inhibition of osteogenesis in hPDLSCs. (A) Western blot assay for ALP, RUNX2 and COL1 protein expression in hPDLSCs after osteogenic induction for 7 days under control, TNF-α, TNF-α (20ng/ml) and triptolide (10− 11M) treatment. (B) Semi-quantitative analysis of ALP protein levels in hPDLSCs. (C) Relative expression of ALP mRNA. (D) Semi-quantitative analysis of RUNX2 protein levels in hPDLSCs. (E) Relative expression of RUNX2 mRNA. (F) Semi-quantitative analysis of COL1 protein levels in hPDLSCs. (G) Relative expression of COL1 mRNA. The blots were cut prior to hybridisation with antibodies during blotting

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BMC Complementary Medicine and Therapies

ISSN: 2662-7671

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