Fig. 4

JPYSF ameliorates necroptosis in cisplatin-induced AKI mice. (a) Representative images of TUNEL staining in the renal tissues (n = 3). Positive cells are visualized as green and normal nuclei are stained with DAPI (blue). (b) Quantitative analysis for the number of TUNEL-staining positive cells. (c) Representative WB images of necroptotic proteins, such as P-RIPK1, P-RIPK3 and P-MLKL in the kidneys. (d) - (f) Densitometric analyses of P-RIPK1, P-RIPK3 and P-MLKL proteins expression normalized to GAPDH content (n = 3). (g) and (h) mRNA expression levels of RIPK3 and MLKL in renal tissues were measured by qRT-PCR. Data presented are means ± SEM. ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001 vs. the control group; ##P < 0.01, ###P < 0.001, ####P < 0.0001 vs. the cisplatin group