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Table 9 Comet assay of genomic DNA of human lymphocytes exposed to chloroformic extract of Chaerophyllum villosum Wall. ex DC. for 24 h

From: HPLC analysis, genotoxic and antioxidant potential of Achillea millefolium L. and Chaerophyllum villosum Wall ex. Dc

Class

Negative Control (Only Lymphocytes)

Positive Control (Lymphocytes + H2O2)

50 mg/100 ml

75 mg/100 ml

100 mg/100 ml

Class 0

92.7 ± 2.5

6.7 ± 2.8

45.7 ± 5.1

73.0 ± 5.0

78.7 ± 2.1

Class 1

3.3 ± 1.5

20.7 ± 9.0

24.3 ± 10.2

15.0 ± 3.0

13.0 ± 2.0

Class 2

1.3 ± 1.2

17.7 ± 7.5

17.3 ± 3.1

6.7 ± 1.5

5.7 ± 0.6

Class 3

2.3 ± 2.5

24.0 ± 5.3

8.0 ± 2.6

4.0 ± 1.7

2.0 ± 1.0

Class 4

0.3 ± 0.6

34.3 ± 4.0

4.7 ± 2.1

1.3 ± 1.1

0.6 ± 0.5

TCS

14.3 ± 8.6

265.3 ± 20.4

101.7 ± 8.6*

45.7 ± 9.0**

33.0 ± 4.3**

  1. Values are expressed as mean ± standard deviation (S.D). Difference significant relative to positive control at *P < 0.002, **P < 0.0001 (One-way ANOVA, Tukey Test)
  2. TCS Total comet score