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Table 5 Comet assay of genomic DNA of human lymphocytes exposed to methanolic extract of Chaerophyllum villosum Wall. ex DC. for 24 h

From: HPLC analysis, genotoxic and antioxidant potential of Achillea millefolium L. and Chaerophyllum villosum Wall ex. Dc

Class

Negative Control (Only Lymphocytes)

Positive Control (Lymphocytes + H2O2)

50 mg/100 ml

75 mg/100 ml

100 mg/100 ml

Class 0

90.0 ± 5.0

8.3 ± 5.7

50.6 ± 7.1

61.7 ± 13.7

73.7 ± 8.5

Class 1

6.0 ± 3.6

21.6 ± 10.4

27.3 ± 8.1

21.0 ± 9.6

16.3 ± 7.7

Class 2

3.0 ± 2.0

21.7 ± 7.6

14.3 ± 2.5

10.0 ± 2.0

6.3 ± 0.6

Class 3

0.3 ± 0.5

18.3 ± 7.6

6.3 ± 3.2

5.0 ± 1.7

2.3 ± 0.6

Class 4

0.6 ± 1.1

26.7 ± 12.6

2.7 ± 2.1

2.3 ± 0.6

1.3 ± 0.6

TCS

15.7 ± 6.6

226.7 ± 41.6

85.7 ± 22.0*

54.7 ± 4.0*

44.0 ± 7.2*

  1. Values are expressed as mean ± standard deviation (S.D). Difference significant relative to positive control at *P < 0.001 (One-way ANOVA, Tukey Test)
  2. TCS Total comet score