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Table 2 Comet assay of genomic DNA of human lymphocytes exposed to methanolic extract of Achillea millefolium L. for 3 h

From: HPLC analysis, genotoxic and antioxidant potential of Achillea millefolium L. and Chaerophyllum villosum Wall ex. Dc

 

Negative Control (Only Lymphocytes)

Positive Control (Lymphocytes + H2O2)

50 mg/100 ml

75mg100ml

100 mg/100 ml

Class 0

90.0 ± 5.0

15.0 ± 5.0

54.3 ± 4.0

76.0 ± 5.3

81.0 ± 6.5

Class 1

4.0 ± 1.7

43.3 ± 11.5

23.3 ± 5.8

10.7 ± 1.2

8.7 ± 1.1

Class 2

3.3 ± 1.5

20.0 ± 13.2

12.3 ± 2.5

6.7 ± 2.8

4.7 ± 0.6

Class 3

1.7 ± 1.1

16.7 ± 7.6

6.0 ± 1.7

4.3 ± 1.5

2.7 ± 2.5

Class 4

1.0 ± 1.0

8.3 ± 2.8

4.0 ± 1.7

2.7 ± 1.1

3.0 ± 2.6

TCS

17.3 ± 13.1

166.7 ± 42.5

78.0 ± 9.6*

47.7 ± 14.1**

38.3 ± 20.1***

  1. Values are expressed as mean ± standard deviation (S.D). Difference significant relative to positive control at *P < 0.01, **P < 0.002, ***P < 0.001 (One-way ANOVA, Tukey Test)
  2. TCS Total comet score