Table 1 Quantitative phytochemical screening of Achillea millefolium L. and Chaerophyllum villosum Wall. ex DC

Achillea millefolium L.

 S.No

Extract

Alkaloids (mg/g)

Phenols (mg/g)

Tannins (mg/g)

Flavonoids (mg/g)

 1

Methanol

27.4

34.5

12.7

32.4

 2

Chloroform

16.6

18.6

14.6

25.6

Chaerophyllum villosum Wall. ex DC.

 1

Methanol

24.7

13.5

26.4

28.3

 2

Chloroform

31.4

19.3

16.6

35.5

A: HPLC analysis of methanol extract of Achellia millefolium

 Peak no

Retention time

Compounds

Area

 1

4.53

Apegenin

12453.21

 2

10.32

Caffiec acid

21543.12

 3

13.65

Kaempferol

42536.42

 4

16.45

Syringic acid

52512.25

 5

22.43

Ferulic acid

35231.13

 6

26.36

Sinapic acid

64372.38

B: HPLC analysis of chloroform extract of Achellia millefolium

 Peak no

Retention time

Compounds

Area

 1

11.42

Quercitrin

24262.41

 2

18.63

Salicylic acid

43241.23

 3

25.53

Cinnamic acid

65362.53

 4

35.34

Apegenin

82142.71

 5

43.51

Rutin

76722.43

C: HPLC analysis of methanol extract of Chaerophyllum villosum

 Peak no

Retention time

Compounds

Area

 1

10.64

Kaempferol

25623.23

 2

16.50

Myricetin

31352.21

 3

24.43

Caffeic acid

54152.32

 4

29.33

Ellagic acid

63143.38

 5

36.42

Catechin

83125.62

 6

39.31

Rutin

96543.41

 7

44.52

Chlorogenic acid

92521.30

D: HPLC analysis of chloroform extract of Chaerophyllum villosum

 Peak no

Retention time

Compounds

Area

 1

12.52

Luteolin

13673.43

 2

23.41

m Coumaric acid

21435.32

 3

30.62

Caffeic acid

42121.52

 4

32.30

Ellagic acid

52174.21

 5

40.24

Ferulic acid

64142.45

 6

44.48

Rutin

72642.61