Table 1 Primers used for real-time PCR
Name of gene | Primer sequence | |
|---|---|---|
IL-1β | Forward | 5`- GTGATGAAAGACGGCACACC-3′ |
Reverse | 5`- TCCTGGGGAAGGCATTAGGA-3′ | |
GAPDH | Forward | 5`-CTCTCTGCTCCTCCCTGTTC-`3 |
Reverse | 5`-CGACATACTCAGCACCAGCA-`3 | |
IL-6 | Forward | 5`-TCTGGTCTTCTGGAGTTCCGT-3′ |
Reverse | 5`-GGATGGTCTTGGTCCTTAGCC-3′ | |
TNF-α | Forward | 5`-CCTCTCTGCCATCAAGAGCC-3′ |
Reverse | 5`- GGCTGGGTAGAGAACGGATG-3′ | |
COX-2 | Forward | 5` TTCGGGAGCACAACAGAGTG 3′ |
Reverse | 5` CAGCGGATGCCAGTGATAGA 3′ | |
iNOS | Forward | 5′- CACCACCCTCCTTGTTCAAC -3′ |
Reverse | 5′- CAATCCACAACTCGCTCCAA -3′ |