Fig. 3

Effect of MEDD on immune cell population in IMQ-induced psoriasis

The dorsal skin of mice was treated with Vaseline, IMQ, IMQ and Vh or MEDD for 7 days, and the immune cell population was analyzed by flow cytometry in the skin draining lymph nodes the next day. a CD4⁺IFNγ⁺ Th1 and CD4⁺ IL-17A⁺ Th17 cell populations were analyzed. (left) Representative dot blot and (right) quantified percentage of Th1 and Th17 cells. b IL-17A⁺ γδTCR⁺ (IL-17 producing γδT cells) cell populations were analyzed. (left) Representative dot blot and (right) quantified percentage of IL-17-producing γδ T cells. These results represent the mean ± S.D. of four independent experiments. Statistical analysis was performed using Student’s t-test. * p < 0.05, compared with mice in the IMQ- and vehicle-treated group; #, p < 0.05; ##, p < 0.01 compared with Vaseline control mice; IMQ, imiquimod; Vh, Vehicle; MEDD, methanol extract of Dictamnus dasycarpus root bark