Fig. 6
From: Inhibitory effects of ChondroT and its constituent herbs on RANKL-induced osteoclastogenesis
Effects of ChondroT and its constituent herbs on RANKL-induced MAPKs activation in BMDMs. a BMDMs were pretreated with ChondroT for 4 h prior to stimulation with 100 ng/mL of RANKL at the indicated time. b Cells were treated with ChondroT or its constituent herbs for 4 h prior to stimulation with 100 ng/mL of RANKL for 30 min. Whole cell lysates were used for Western blot analysis. Phosphorylated MAPKs were detected with antibodies against phospho-ERK, phospho-JNK or phospho-p38. Membranes were stripped and reassessed with antibodies targeting non-phosphorylated MAPKs