Fig. 1

Attenuated CHS responses by hispidulin treatment. a The experimental design for induction of CHS as mentioned in Materials and Methods. Hispidulin at concentrations of either 10 or 30 μg/ear (His10 and His30) or 30 μg/ear dexamethasone (Dex) was applied to both ears once daily on days 4–6. Mice applied with vehicle (Veh) alone were used as a non-sensitized control. b Measurement of ear thickness. Thickness was measured using a Vernier caliper at 6-, 24-, and 48-h post-challenge. All values are presented as mean ± standard deviation (n = 5; *p < 0.05, **p < 0.01, ***p < 0.001)