Fig. 3

a Expression of ENV gene in DENV infected cell after treated with mushroom extract. b Expression of NS5 gene in DENV infected cell after treated with mushroom extract. DENV-2 infected Vero cells were treated with 2000 μg/ml mushroom extracts according to the protocol of simultaneous, attachment and penetration assay. After 48 h post infection, supernatants were collected to extract viral RNA of the mock infected cells, infected cells and treated infected cells. RT-qPCR was carried out to access the expression of ENV and NS5 gene. Gene expression was calculated using the algorithm provided by Bio-Rad CFX Manager Software 1.6. Results were normalized by GAPDH expression and were presented as relative normalized fold expression to virus infected cells which was defined as 1. Data are expressed as mean normalised fold expression ± SEM of two independent experiment (n = 2). Statistical differences compared to mock infected cells are noted with asterisk (*P < 0.05) or (**P < 0.01)