Table 1 Primers of Q-PCR
From: Costunolide induces mitochondria-mediated apoptosis in human gastric adenocarcinoma BGC-823 cells
Gene | Primer | Sequence (5′-3′) | Product (bp) |
|---|---|---|---|
GAPDH | Forward Reverse | GCAAATTCCATGGCACCGTC GACTCCACGACGTACTCAGC | 133 |
Bax | Forward Reverse | GAACCATCATGGGCTGGACA GCGTCCCAAAGTAGGAGAGG | 102 |
Bcl-2 | Forward Reverse | GAACTGGGGGAGGATTGTGG CCGTACAGTTCCACAAAGGC | 183 |
puma | Forward Reverse | GGAGACAAGAGGAGCAGCAG GGTAAGGGCAGGAGTCCCAT | 82 |
Bak1 | Forward Reverse | CACAGAGGAGGTTTTCCGCA ATAGCGTCGGTTGATGTCGT | 178 |