Fig. 2

The effect of OM on miR-195, Smad7 and α-SMA expression in TGF-β1 induced HSC-T6. HSC-T6 was treated with OM (125 μg/mL, 250 μg/mL and 500 μg/mL) for 1 h, and then incubated with TGF-β1 for 24 h. a Real-time PCR analysis for mRNA levels of miR-195 in HSC-T6 treated with OM and TGF-β1. b Real-time PCR analysis for mRNA levels of Smad7 in HSC-T6 treated with OM and TGF-β1. c Real-time PCR analysis for mRNA levels of α-SMA in HSC-T6 treated with OM and TGF-β1. d Western blot analysis for protein expression of α-SMA in HSC-T6 treated with OM and TGF-β1. e Western blot analysis for protein expression of Smad7 in HSC-T6 treated with OM and TGF-β1. n = 3, Data is expressed as mean values (± SEM) in three independent experiments.*P < 0.05 vs control, **P < 0.01 vs control, ^##P < 0.01 vs TGF-β1. ^&& P < 0.01 vs + OM (125 μg/mL)