Fig. 3

a TJFs occurs cell cycle progression. Cell cycle arrest effect was measured by flow cytometry. b Cyclin E and p-Cdk2 Protein levels were determined by Western blot analysis. The β-actin probe served as protein-loading control. c Measurement of Type І 5α-reductase through ELISA assay. TJFs decreased Type І 5α-reductase in HDPs. The statistical analysis of the data was carried out by use of an independent sample ANOVA test. ^*p < 0.05, ^**p < 0.01 and ^***p < 0.001 vs. con (24 h). ^###p < 0.001 (each experiment, n = 3). NS, not significant