Fig. 4

Immunohistochemistry for doublecortin (DCX) in the dentate gyrus of the control group (a and b), dimethylmercury (MeHg)-treated group (c and d), Dendropanax morbifera leaf extract treated group with MeHg (MeHg+DML) (e and f), and galantamine (GAL) treated group with MeHg (MeHg+GAL) (g and h). In the control group, DCX immunoreactive neuroblasts are abundantly in the dentate gyrus. Note that DCX immunoreactive neuroblasts are few and their dendrites are poorly developed in the MeHg-treated group. In the MeHg+DML-treated group, DCX immunoreactive neuroblasts are abundant and have well-developed dendrites. GCL, granule cell layer; ML, molecular layer; PoL, polymorphic layer. Scale bar = 100 μm (a, c, e, and g), 50 μm (b, d, f, and h). (i and j): Quantitative analysis of DCX immunoreactive neuroblasts. The number per section and the relative optical densities (RODs), expressed as a percentage of the value representing the DCX immunoreactivity in the dentate gyrus of the control group, are also shown (n = 5 per group; ap < 0.05, vs. control group; bp < MeHgtreated group). Values are mean ± standard error of the mean