Fig. 4

Inhibitory effects of crude microcystins on mRNA expression level of VEGFR-2, PI3 K, β-Catenin, VE-Cadherin, Akt, and NF-kB analysed by RT-PCR b. The possible mechanisms of the inhibition of angiogenesis by the crude microcystins. We supposed and graphically described the mechanism of inhibition of angiogenesis by crude microcystin. 500 μl microsytins were isolated from 10 ml M. aeruginosa culture (OD660 = 1.8) and then different volumes of the crude microcystins were (5–60 μl/mL) used for the antiangiogenic assay