Fig. 5

Effect of SHW administration on type II collagen IgG levels and infiltration of inflammatory cells in the synovial membrane. Mice were separated into 5 groups (Control; n = 7, CIA; n = 7, CIA + MTX; n = 7, CIA + 100 mg/kg SHW; n = 7, CIA + 500 mg/kg SHW; n = 7). (a) Type II collagen IgG in serum was analyzed using ELISA. (b) Representative images were stained with hematoxylin and eosin (H&E). Infiltration of inflammatory cells is indicated with black arrowheads. (c) Histological scores were determined on H&E stained sections in different groups of mice. Histologic scoring of inflammation and bone erosion was performed by three independent observers. Data are presented as the mean ± SEM (n = 7). ^#p < 0.05, versus normal control group; ^*p < 0.05, versus CIA group. Between group comparisons were conducted using one-way ANOVA with Tukey’s post hoc test. CIA, collagen induced arthritis; MTX, methotrexate; SHW, water extract of Saururus chinensis leaves