Fig. 3

Inhibitory effect of ES extract on mushroom tyrosinase activity, B16F10 melanin content and intracellular tyrosinase activity. a Different concentrations of the ES extract (8, 40, 200 μg/ml) or arbutin (10 mM) was incubated with the same units of mushroom tyrosinase. Following incubation, the amount of dopachrome produced was determined at 490 nm. b and c B16F10 melanoma cells were stimulated with α-MSH (100 nM) for 48 h, and then the melanin content or intracellular tyrosinase activity were measured after treatment with various concentrations of the ES extract (8, 40,200 μg/ml) or arbutin (10 mM) for another 24 h. Results are represented as percentages of control, and data are presented as mean ± S.D. for three separate experiments. Values are significantly different by comparison with control. *: p < 0.05, **: p < 0.01 α-MSH treatment alone