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Fig. 4 | BMC Complementary and Alternative Medicine

Fig. 4

From: Comparison of the main components and bioactivity of Rhus verniciflua Stokes extracts by different detoxification processing methods

Fig. 4

Effect of DRVE, FRVE, FFRVE on cellular lipid accumulation and lipogenesis in OA-induced HepG2 cells. Cells were co-treated with OA and 400 μg/ml of DRVE, FRVE, FFRVE for 24 h. a Cells were stained with ORO as described in the materials and methods and then quantitatively analyzed. ORO staining image (magnification 400×), (##) p < 0.01 (in comparison to the non-OA treated control), * and ** represent significant differences (p < 0.05 and p < 0.01, respectively) with respect to the OA-treated control. b Determination of the expression of AMPK, SREBP-1 and PPARα by western blotting analysis. Quantitative protein levels were shown. (##) p < 0.01, and (###) p < 0.001 (in comparison to the non-OA-treated control). () p < 0.01 and () p < 0.001 (in comparison to the OA-treated control). c Total intracellular TG was analyzed by an enzymatic colorimetric method. Data are represented as the mean ± SD of three experiments. (###) p < 0.001 (in comparison to the non-OA treated control). () p < 0.01 and () p < 0.001 (in comparison to the OA-treated control)

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