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Fig. 2 | BMC Complementary and Alternative Medicine

Fig. 2

From: Sotetsuflavone inhibits proliferation and induces apoptosis of A549 cells through ROS-mediated mitochondrial-dependent pathway

Fig. 2

Sotetsuflavone induced apoptosis in A549 cells. a Fluorescence observation after treating A549 cells with sotetsuflavone using Annexin V-FITC/PI double staining assay (100 × magnification). b Effect of sotetsuflavone on A549 cell apoptosis rate (early and late apoptotic cells) after treating A549 cells with sotetsuflavone. c Fluorescence image analysis of apoptotic A549 cells induced with sotetsuflavone by using Annexin V-FITC/PI double staining, where quadrant Q3 are early apoptotic cells, and Q2 are late apoptotic or necrotic cells. The results from three independent experiments were expressed as mean ± SD compared with the control group, ***P < 0.001

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