Fig. 3

The effect of TY-NS-B on the cell growth and cyclin D1 expression in other cancer cells. a Human breast cancer cells (MDA-MB-231), human pancreatic cancer cells (AsPC-1), human non-small cell lung cancer cells (A549) and human prostate cancer cells (PC-3) were plated overnight and then treated with TY-NS-B at the indicated concentrations for 24 h. Cell growth was measured using MTT assay. Data represent mean ± SD for three independent experiments. *P < 0.05 compared to cell without TY-NS-B. b MDA-MB-231, AsPC-1, A549 and PC-3 were plated overnight and then treated with TY-NS-B at the indicated concentrations for 24 h. Cell lysates were subjected to SDS-PAGE and the Western blot was performed using antibody against cyclin D1. Actin was used as internal control for Western blot analysis