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Table 1 The primers of gene amplification

From: Synergistic effect of phytochemicals on cholesterol metabolism and lipid accumulation in HepG2 cells

GENE Sense Anti-sense Product
ABCA1 5’-AGGCCCAGACCTGTAAATGC-3’ 5’-CTCACCAACCTTGCCAACTTC-3’ 450 bp
SREBP2 5’-CAGACGCCAAGATGCACAAG-3’ 5’-TGGCTCATCTTTGACCTTTGC-3’ 323 bp
CYP7A1 5’-CATTTGGGCACAGAAGCATTG-3’ 5’-AGGCAGCGGTCTTTGAGTTAG-3’ 174 bp
NR1H3 (LXR α) 5’-AAGAAACTGAAGCGGCAAGA-3’ 5’-AGCAATGAGCAAGGCAAACT-3’ 555 bp
PRKAA2 (AMPK α2) 5’-TCAATCGTTCTGTCGCCAC-3’ 5’-ATACGGTTTGCTCTGACTTCG-3’ 530 bp
HMGCR 5’-CTCCGCAGGCTATTTGTTCAG-3’ 5’-GCTAAGAGCGTTCGTGGGTC-3’ 620 bp
GAPDH 5’-ACTCCTCCACCTTTGACGCTG-3’ 5’-CTCTCTTCCTCTTGTGCTCTTGC-3’ 182 bp