Fig. 4

Analysis of protein expression. a Western blot analysis of procaspase-3, cleaved caspase3, caspase-8, Bax, Bcl-2 and Bcl-XL, p-Akt, Akt, p-GSK3β and GSK3β, PI3K, p-PI3K, MMP2 and MMP9. βactin was used to check equal loading. b The scratch assay shows the inhibition of cell invasion into the wound region after 24 h of GAE treatment (1 mg/ml). c The clonogenic assay result shows the inability of single cells to proliferate as a colony after GAE treatment (1 mg/ml). (i) Colony formed in control cells after 7 days, (ii) magnified colony of control cells, (iii) Treated cells after 7 days, (iv) treated cells magnified. C-control, T-treated