Table 1 PCR primer sequences for TGF-β1, Smad2, Smad3, SnoN and GAPDH
Gene | Forward sequence | Reverse sequence |
|---|---|---|
TGF-β1 | 5’-ATACGCCTGAGTGGCTGTCT-3’ | 5’-TGGGACTGATCCCATTGATT-3’ |
Smad2 | 5’-TGAGCTTGAGAAAGCCATCA-3’ | 5’-TGTGTCCCACTGATCTACCG-3’ |
Smad3 | 5’-CATTACCATCCCCAGGTCAC-3’ | 5’-CGTAACTCATGGTGGCTGTG-3’ |
SnoN | 5’-CCATTCAATGCCCCATCCT-3’ | 5’-AGTTCGTGGCCGCAATAAAG-3’ |
GAPDH | 5’-CCTGCCAAGTATGATGACATCAAGA-3’ | 5’-GTAGCCCAGGATGCCCTTTAGT-3’ |