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Table 1 PCR primer sequences for TGF-β1, Smad2, Smad3, SnoN and GAPDH

From: Herbal compound 861 prevents hepatic fibrosis by inhibiting the TGF-β1/Smad/SnoN pathway in bile duct-ligated rats

Gene Forward sequence Reverse sequence
TGF-β1 5’-ATACGCCTGAGTGGCTGTCT-3’ 5’-TGGGACTGATCCCATTGATT-3’
Smad2 5’-TGAGCTTGAGAAAGCCATCA-3’ 5’-TGTGTCCCACTGATCTACCG-3’
Smad3 5’-CATTACCATCCCCAGGTCAC-3’ 5’-CGTAACTCATGGTGGCTGTG-3’
SnoN 5’-CCATTCAATGCCCCATCCT-3’ 5’-AGTTCGTGGCCGCAATAAAG-3’
GAPDH 5’-CCTGCCAAGTATGATGACATCAAGA-3’ 5’-GTAGCCCAGGATGCCCTTTAGT-3’
  1. GADPH, glyceraldehyde-3-phosphate dehydrogenase; Smad2, Sma- and Mad-related protein 2; Smad3, Sma- and Mad-related protein 3; SnoN, ski-related novel protein N; TGF-β1, transforming growth factor-β1