Fig. 1

Gigantol suppresses the activity of the Wnt signaling pathway. a Structure of gigantol. b-e HEK293T cells were transfected with SuperTopFlash reporter gene together with control vector or expression plasmids encoding Wnt1 (b), LRP6 (c), Wnt1 and LRP6 (d), and β-catenin (e). After transfection for 24 h, the transfected cells were treated with DMSO or the indicated concentrations of gigantol for 24 h (f). The SuperTopFlash reporter gene was transfected into HEK293T cells. At 24 h after transfection, cells were incubated with the control or Wnt3A-CM containing DMSO or the specified concentrations of gigantol for 24 h. The luciferase values were normalized using β-gal activities. Statistical significance between groups is calculated by two-way ANOVA, *P < 0.05 compared to cells transfected with Wnt1, LRP6, or Wnt1 and LRP6, or incubated with Wnt3A-CM containing DMSO