Table 3 Apoptotic lymphocytes detection by binding the surface of cells with annexin V and staining with PI under the administration of Galega officinalis extract in normal conditions and under experimental diabetes mellitus
Cell viability, % | Â | |||
|---|---|---|---|---|
C | C + G | D | D + G | |
Viable cells (annexin V−/ PI−) | 96,41 ± 1,70 | 99,51 ± 0,07 | 87,56 ± 1,12* | 98,12 ± 0,26# |
Cells with features of early apoptosis (annexin V+/PI−) | 2,75 ± 1,92 | 0,11 ± 0,04* | 10,38 ± 0,58* | 1,39 ± 0,31# |
Cells with features of late apoptosis (annexin V+/PI+) | 0,06 ± 0,02 | 0,004 ± 0,002 | 0,16 ± 0,08* | 0,04 ± 0,02# |
Cells with features of necrosis (annexin V−/PI+) | 0,78 ± 0,30 | 0,38 ± 0,06* | 1,91 ± 1,76 | 0,44 ± 0,07# |