Fig. 5

MOP (a) and NOP (b) relative gene expression in SH-SY5Y neuroblastoma cells after 5 h of 100 μM naloxone and WSE exposure (0.25 mg/ml, 0.50 mg/ml and 1.00 mg/ml). Data represent 2^−DDCt values calculated by using the DDCt method. Gene expression was normalized to GAPDH as housekeeping gene. Data are expressed as mean ± SEM (**p < 0.01, ***p < 0.001 vs vehicle, #p < 0.05, ##p < 0.01 vs naloxone +1.00 mg/ml WSE; data analyzed by one-way ANOVA followed by Newman-Keuls tests)