Fig. 4

Inhibitory effect of DHSMT on triglyceride (TG) and leptin accumulation in 3T3-L1 adipocytes. 3T3-L1 preadipocytes were differentiated into adipocytes by using a mixture of 3-isobutyl-1-methylisobutylxanthine, dexamethasone and insulin (MDI) with various concentrations of DHSMT (0, 62.5, 125, 250 and 500 μg/ml) and SB203580 for 7 days. a TG content was measured enzymatically by using TG assay kit (Bioassay Systems, CA, USA). b Leptin production was measured at 450 nm by using a mouse leptin immunoassay kit (R&D Systems). The data are mean values of three experiments ± SEM; ## < 0.01 compared with control, *P < 0.05; **P < 0.01 compared with the MDI