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Table 1 Effects of PM21 on cAMP level, TXB2 formation, serotonin release, and intracellular calcium concentration

From: Antiplatelet mechanism of an herbal mixture prepared from the extracts of Phyllostachys pubescens leaves and Prunus mume fruits

Treatment (μg/mL)

cAMP level (pmol/109 platelets)

TXB2 concentration (pg/mL)

Serotonin concentration (ng/mL)

Intracellular Ca2+ concentration (nM)

Collagen

Control

PM21

IBMX

    

0.48 ± 0.12

64.63 ± 63.88

55.25 ± 31.86

139.80 ± 13.70

5

DMSO

  

–

777.76 ± 55.04

650.46 ± 38.05

2010.70 ± 337.60

5

 

200

 

4.71 ± 0.39***

347.50 ± 48.76***

369.99 ± 18.64***

1288.20 ± 310.20

5

 

100

 

4.35 ± 0.13***

468.93 ± 56.76**

476.14 ± 53.98**

836.50 ± 88.70**

5

 

50

 

–

651.07 ± 84.75

358.18 ± 98.73**

640.70 ± 153.80**

5

Aspirin (50 μg/mL)

  

6.81 ± 0.73***

389.79 ± 17.00***

369.99 ± 20.26***

632.30 ± 69.20***

5

  

50

2.61 ± 0.16***

   

5

 

200

50

5.59 ± 0.25***

   

5

 

100

50

4.89 ± 0.59**

   

5

Aspirin (50 μg/mL)

 

50

8.11 ± 1.45**

   
  1. Effects of PM21 on cAMP level, thromboxane B2 formation and serotonin release. Rat platelets were pre-incubated for 2 min with PM21 (100 and 200 μg/mL) or aspirin (50 μg/mL) with or without IBMX (50 μg/mL). 0.1% (v/v) DMSO was used for vehicle. Then they were stimulated with 5 μg/mL collagen in the presence of Ca 2+ (1 mM) for 5 min. After termination of aggregation reactions, the samples were centrifuged and cAMP level of supernatants was determined with cAMP EIA Kit. Rat platelets were pre-incubated for 2 min with PM21 (50, 100, 200 μg/mL) or aspirin (50 μg/mL), and stimulated with 5 μg/mL collagen in the presence of Ca 2+ (1 mM) for 5 min. After termination of aggregation reactions, the samples were centrifuged, and TXB2 and serotonin concentrations of supernatants were determined with TXB2 EIA kit and serotonin ELISA kit, respectively. Effects of PM21 on intracellular calcium concentration. Rat platelets were incubated with 5 mM Fura-2/AM for 60 min at 37 °C. The Fura-2-loaded platelets were then pre-incubated for 2 min with PM21 (50, 100, 200 μg/mL) or aspirin (50 μg/mL), and stimulated with 5 μg/mL collagen in the presence of Ca 2+ (1 mM) for 5 min. Fura-2 fluorescence was measured by spectrofluorometer at the emission wavelength of 510 nm. From the spectrofluorometric measurements, intracellular concentration of calcium ion was calculated. Data show the mean ± SEM of at least three independent experiments. *** p < 0.001 versus vehicle control
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BMC Complementary Medicine and Therapies

ISSN: 2662-7671

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