Fig. 5
Effects of PM21 on phosphorylation of ERK2 and p38. Rat platelets were pre-incubated for 2 min with PM21 (50, 100, 200 μg/mL) or aspirin (50 μg/mL), and stimulated with 5 μg/mL collagen in the presence of Ca2+ (1 mM) for 5 min. After termination of aggregation reactions, total cell proteins were extracted. The proteins were separated by SDS-PAGE and transferred on to nitrocellulose membranes. The membranes were then probed with antibodies against phospho-ERK2, ERK2, phospho-p38, p38 and β-actin. Antibody binding was visualized by chemiluminescence. All immunoblots are representatives of three or four independent experiments