Fig. 2

Mitosis and cell cycle in metastatic tissues. a Mitosis in the spleen, liver and other sites in mice with metastatic tumors. Black bars: numbers of mitotic figures in spleen, gray bars: numbers of mitotic figures in liver, open bars: numbers of mitotic figures in other abdominal tissues including skin and smooth muscle, adipose and mesentery lymph node. Y-axis is the number of mitotic figures per 20× power field. b Immunohistochemical staining of phosphorylated histone H3 serine 10 (H3S10p) in the metastatic liver. The upper panels show 20× power field and the lower panels show 10× power field of cross-sectioned mouse liver. The dotted lines outline tumor area without necrosis. c Quantification of H3S10p staining in the metastatic liver. Y-axis represents the number of positive cells per 20X power field. d Cell cycle analysis in mouse liver with metastasis. X-axis represents the signal intensity by PI staining and Y-axis indicates the total cells count. e Quantification of cell cycle analysis. Y-axis represents the percentage of gated cells (%). Open bars: % of cells in G0/G1 phase, grey bars: % of cells in the S phase, black bars: % of cells in the G2/M phase. f Immunohistochemical staining of Ki67 in the metastatic liver. The upper panels show 20× power field and the lower panels show 10× power field of cross-sectioned mouse liver. The dotted lines outline tumor area without necrosis. g Quantification of Ki67 staining in the metastatic liver. Y-axis represents the percentage of proliferating cells (%). Asterisks (*) indicate statistical significance (n > 4, p <  0.05) compared to PBS group