Fig. 2

Fluorescence intensity (a) and mean intensity (b) following 3 h of treatment with insulin (positive control), A. karroo (AK) and insulin + A. karroo (AK + I) and untreated C2C12 cells (standard control). a 1 = green fluorescence represent FITC bound GLUT4 molecules, 2 = blue represent Wuchi stained nucleus, 3 = Overlay of images 1 and 2 and 4 = intensity surface plot. All the images were taken at a magnification of 40X. b The results obtained were expressed as means ± SEM. The statistical significance of the results was tested using one way ANOVA, employing the Tukey-Kramer multiple comparisons test. The P value significance was represented by an asterisk (*) for P < 0.05, two asterisks (**) for P < 0.01 and three asterisks (***) for P < 0.001