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Fig. 5 | BMC Complementary and Alternative Medicine

Fig. 5

From: Ethanol extract of propolis and its constituent caffeic acid phenethyl ester inhibit breast cancer cells proliferation in inflammatory microenvironment by inhibiting TLR4 signal pathway and inducing apoptosis and autophagy

Fig. 5

EECP and CAPE induced apoptosis and autophagy in LPS-stimulated MDA-MB-231 cells. a The expression of caspase 3, procaspase 3 and PARP were detected by western blotting at 24 h. b Quantification of relative expression of procaspse 3, caspase 3 and PARP. c Cells were stained with anti-LC3B antibody for immunostaining. Immunofluorescence graphs showed an increase of endogenous punctuate LC3B. d The expression of LC3B and p62 were detected by western blotting at 24 h. e Quantification of relative expression of LC3-II, LC3-II/ LC3-Iand p62 in LPS-stimulated MDA-MB-231 cells. (* P < 0.05, ** P < 0.01 vs control, n = 3). Data are means ± S.E.M

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