Fig. 2
TTF protects neuronal cells from Aβ-induced cell death. Neuronal cells were treated with Aβ25–35 at 25 μM, and cell death was determined 20 h later by (a) the LDH and (b) the crystal violet methods. Neuronal cells were treated with various concentrations of TTF, Aβ was added and cell death was determined 20 h later by (c) the LDH and (d) the crystal violet methods. The results are means ± SEM of two experiments (n = 16); *** P < 0.001, for comparison of treated with untreated cells. The maximal LDH release after disruption of cells by Triton x-100 was A492 = 0.61 ± 0.04 as measured in two experiments (n = 5). (e) Cells were treated with TTF alone, at various concentrations, and cell viability was determined 20 h later by the crystal violet method. The results are means ± SEM of two experiments (n = 16). *** P < 0.001, for comparison with cells that were treated with Aβ alone